SIRT-1与SUMO-1在子宫内膜癌中的表达及其临床意义

发布时间：2018-03-06 16:35

本文选题：SIRT-1　切入点：SUMO-1　出处：《青岛大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:检测SIRT-1(Silent information regulator 1)与SUMO-1(Small ubiquitin-related modifier-1)在不同子宫内膜组织中的表达情况并推测其与内膜癌发生发展之间可能的内在联系。方法:收集并保存2014年10月1日至2016年06月30日在青岛大学附属医院妇科与青岛市妇女儿童医院妇科行手术切除后并经病理确诊的73例异常子宫内膜组织,其中子宫内膜癌组织45例(均为腺癌)、子宫内膜不典型增生组织28例,同时选择因单纯子宫肌瘤行全子宫切除术后并经病理确诊为正常子宫内膜标本30例。应用免疫组织化学方法及western-blot方法分别检测SIRT-1蛋白与SUMO-1蛋白在各组子宫内膜组织中的表达水平,利用统计学方法统计并分析其表达程度与子宫内膜病变程度的关系以及其与子宫内膜癌组织各项临床病理参数之间的相关关系。结果:45例子宫内膜癌患者年龄区间为35~73岁(52.85±8.41),其中50岁32例,占71.1%,≤50岁13例,占28.8%;(1)SIRT-1蛋白的阳性表达率在正常子宫内膜组织、子宫内膜不典型增生组织、子宫内膜癌组织分别为6.67%、42.9%,62.2%且呈递增趋势,差异有统计学意义(P0.05);(2)SUMO-1蛋白的阳性表达率在正常子宫内膜组织、子宫内膜不典型增生组织、子宫内膜癌组织分别为6.67%、32.1%、55.6%且呈递增趋势,差异有统计学意义(P0.05);(3)两者共同阳性表达率在正常子宫内膜组织、子宫内膜不典型增生组织、子宫内膜癌组织组中分别为0%、25.0%、42.2%且呈递增趋势,差异有统计学意义(P0.05);(4)两者各自在有淋巴结转移组的阳性表达率明显高于与淋巴结转移阴性组(P0.05);且两者各自与子宫内膜癌组织学分化程度、淋巴结转移及FIGO临床分期有关(P0.05);而与内膜癌分型、年龄无关(P0.05)。结论:SIRT-1与SUMO-1在子宫内膜组织中的阳性表达率各自随着正常子宫内膜组织、子宫内膜不典型增生组织、子宫内膜癌的发展过程表现出增高趋势,且与内膜癌的淋巴结转移程度、子宫内膜癌组织学分化程度、以及临床分期相关,同时两者在子宫内膜癌的发生发展过程中可能起到重要协同作用,有望成为子宫内膜癌基因治疗的新途径。
[Abstract]:Objective: to detect the expression of SIRT-1(Silent information regulator 1 (SIRT-1(Silent information regulator 1) and SUMO-1(Small ubiquitin-related modifier-1 (SUMO-1(Small ubiquitin-related modifier-1) in different endometrial tissues and to speculate their possible relationship with the development of endometrial carcinoma. Methods: from October 1st 2014 to June 30th 2016, the expression of SIRT-1(Silent information regulator 1 and SUMO-1(Small ubiquitin-related modifier-1 were collected and preserved. 73 cases of abnormal endometrial tissue were surgically resected and pathologically confirmed in the Gynecology Department of Qingdao University affiliated Hospital and Qingdao Women and Children's Hospital. There were 45 cases of endometrial carcinoma (all adenocarcinoma, 28 cases of atypical hyperplasia of endometrium). 30 cases of normal endometrium were selected after hysterectomy because of simple uterine leiomyoma and confirmed by pathology. SIRT-1 protein and SUMO-1 protein were detected by immunohistochemical method and western-blot method respectively in each group of endometrium group. The level of expression in weaving, The relationship between the expression of endometrial carcinoma and the degree of endometrial lesion and the clinicopathological parameters of endometrial carcinoma was analyzed by statistical method. Results the age area of 45 patients with endometrial carcinoma was analyzed. Among them, 32 cases were 50 years old. The positive expression rate of SIRT-1 protein in normal endometrium, atypical hyperplasia of endometrium and endometrial carcinoma was 6.67%, 42.9% and 62.2%, respectively. The positive expression rate of SUMO-1 protein in normal endometrium, atypical hyperplasia of endometrium and endometrial carcinoma was 6.675.1and 55.6%, respectively, and the positive rate of SUMO-1 protein was increasing in normal endometrium, endometrial atypical hyperplasia and endometrial carcinoma, respectively, and the positive expression rate of SUMO-1 protein in normal endometrium, endometrial atypical hyperplasia and endometrial carcinoma was significantly higher than that in normal endometrium. There was significant difference in the positive rate of the two groups (P 0.05). The positive rates of the two groups in normal endometrium, atypical hyperplasia of endometrium and endometrial carcinoma were 42.2% and 42.2%, respectively. The positive expression rate of the two groups in the group with lymph node metastasis was significantly higher than that in the group with negative lymph node metastasis (P 0.05), and the degree of histological differentiation between the two groups and the histological differentiation of endometrial carcinoma was significantly higher than that in the group with negative lymph node metastasis. Lymph node metastasis and clinical stage of FIGO were related to P0.05, but age was not related to the type of endometrial carcinoma. Conclusion the positive expression rates of SUMO-1 and SIRT-1 in endometrial tissue were different with normal endometrium and atypical hyperplasia of endometrium. The development of endometrial carcinoma shows an increasing trend, which is related to the degree of lymph node metastasis, histological differentiation, and clinical stage of endometrial carcinoma. At the same time, they may play an important synergistic role in the development of endometrial carcinoma, which may be a new way of gene therapy for endometrial carcinoma.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.33

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