槟榔碱对口腔癌患者及小鼠肝脏功能影响的初步研究

发布时间：2018-03-08 22:02

本文选题：槟榔碱　切入点：口腔癌　出处：《口腔医学研究》2017年06期 　论文类型：期刊论文

【摘要】：目的:通过比较有、无槟榔咀嚼史的口腔癌患者肝功能指标变化,并与槟榔碱诱导小鼠口腔黏膜下纤维化模型的肝脏损伤比较,探讨槟榔碱对肝脏功能的影响。方法:比较有、无咀嚼槟榔史各50名口腔癌男性患者谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)、总胆红素(TBIL)肝功能指标差异;将45只小鼠,随机分成对照组、停药组、不停药组,每组15只。配制1000mg/L槟榔碱溶液分别持续自由喂养停药组、不停药组2周后,停药组改用实验室灭菌水自由喂养2周,不停药组继续槟榔碱溶液喂养2周;对照组用实验室灭菌水自由喂养4周。检测各组小鼠不同时间段上述肝功能指标变化情况,并采用苏木素-伊红(HE)染色观察小鼠肝脏组织学改变。结果:与无咀嚼槟榔史患者相比,咀嚼槟榔的肝功能指标AST、TBIL显著升高(P0.05),ALT、ALP无明显变化;与对照组小鼠相比,停药组和不停组随用药时间增长,ALT、AST、ALP显著升高(P0.05),而停药组停药2周后ALT、AST、ALP显著下降(P0.05),TBIL无统计学差异;HE染色显示肝脏均出现损伤,但不停药组小鼠损伤更为明显。结论:槟榔的成分对肝脏有损伤,其作用的有效成分可能是槟榔碱。
[Abstract]:Objective: through the comparison, no change of hepatic function index of oral cancer and areca chewing history, arecoline induced liver injury mouse model of oral submucous fibrosis compared to investigate the effects of arecoline on liver function. Methods: compared with areca, without chewing each 50 history of oral cancer in male patients with alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total bilirubin (TBIL) between the index of liver function; 45 mice were randomly divided into control group, withdrawal group, stop drug group, 15 rats in each group. The preparation of 1000mg/L solution were continuously fed with arecoline withdrawal group, after 2 weeks of non-stop the medicine group, withdrawal group with laboratory water during the 2 weeks of feeding, stop the drug group to arecoline 2 weeks feeding solution; the control group with sterile water free laboratory diet for 4 weeks. Mice were detected at different time the changes of liver function, and the hematoxylin Eosin (HE) staining was used to observe the pathological changes of liver tissue of mice. Results: compared with patients without chewing areca history, liver function index AST chewable areca, TBIL significantly increased (P0.05), ALT, ALP did not change significantly; compared with the control group mice, withdrawal group and the group with the drug does not stop growth, ALT, AST, ALP were significantly increased (P0.05), and withdrawal group 2 weeks after the withdrawal of ALT, AST, ALP decreased significantly (P0.05), there was no significant difference in TBIL; HE staining showed that the liver was damaged, but not stop drug group injury in mice was more obvious. Conclusion: areca component damage to the liver. Its effective ingredients may be arecoline.

【作者单位】：广西医科大学口腔医学院;广西医科大学第一附属医院口腔科;海南省人民医院口腔科;
【基金】：国家自然科学基金(编号:81460105) 海南省自然科学基金资助项目(编号:20158321) 海南省卫生厅课题资助项目(编号:14A210189)
【分类号】：R739.8

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