MEG3和MALAT-1 lncRNA与垂体生长激素腺瘤发展及侵袭的关系

发布时间：2018-03-09 09:35

本文选题：生长激素腺瘤　切入点：lncRNA　出处：《广东医学》2017年03期 　论文类型：期刊论文

【摘要】：目的探索MEG3和MALAT-1长链非编码RNA(lncRNA)的表达与垂体生长激素(GH)腺瘤发展和侵袭的关系。方法应用qRT-PCR检测MEG3和MALAT-1 lncRNA在正常垂体前叶、非侵袭性垂体GH腺瘤、侵袭性垂体GH腺瘤的表达情况,分析其表达差异。结果 MEG3 lncRNA在12例(30.8%)垂体GH腺瘤中表达缺失,在正常垂体前叶无表达缺失。MEG3 lncRNA在侵袭性垂体GH腺瘤(P0.01)和非侵袭性垂体GH腺瘤(P0.01)中的表达水平较正常垂体前叶明显降低。MEG3 lncRNA在侵袭性垂体GH腺瘤中的表达水平较非侵袭性垂体GH腺瘤明显降低(P0.01)。MALAT-1 lncRNA在侵袭性垂体GH腺瘤(P0.01)和非侵袭性垂体GH腺瘤(P0.01)中的表达水平较正常垂体前叶明显升高。MALAT-1 lncRNA在侵袭性垂体GH腺瘤中的表达水平较非侵袭性垂体GH腺瘤明显升高(P0.01)。结论 MEG3 lncRNA的表达缺失与MALAT-1的高表达可能与垂体GH腺瘤的发展和侵袭密切相关。
[Abstract]:Objective to investigate the relationship between the expression of MEG3 and MALAT-1 and the development and invasion of pituitary growth hormone (GH) adenoma. Methods qRT-PCR was used to detect the expression of MEG3 and MALAT-1 lncRNA in normal pituitary anterior lobe and noninvasive pituitary GH adenoma. Results the expression of MEG3 lncRNA was absent in 12 cases of pituitary GH adenoma. The expression level of MEG3 lncRNA in invasive pituitary GH adenoma (P0.01) and noninvasive pituitary GH adenoma (P0.01) was significantly lower than that in normal pituitary anterior lobe. MEG3 lncRNA expression level in invasive pituitary GH adenoma was significantly lower than that in normal pituitary anterior lobe. Non-invasive pituitary GH adenoma significantly decreased the expression level of P0.01-MALAT-1 lncRNA in invasive pituitary GH adenoma (P0.01) and non-invasive pituitary GH adenoma (P0.01). The expression of MALAT-1 lncRNA in invasive pituitary GH adenoma was significantly higher than that in normal pituitary anterior lobe. Conclusion the loss of MEG3 lncRNA expression and the high expression of MALAT-1 may be closely related to the development and invasion of GH pituitary adenoma.
【作者单位】：首都医科大学附属北京天坛医院神经外科;北京市神经外科研究所;
【基金】：国家自然科学基金资助项目(编号:81271522,81601205) 国家高技术研究发展计划(863计划)项目(编号:2014AA020610)
【分类号】：R736.4

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