葡萄糖对卵巢癌细胞生长增殖影响体外研究

发布时间：2018-03-10 08:26

本文选题：卵巢癌　切入点：葡萄糖　出处：《中华肿瘤防治杂志》2017年10期 　论文类型：期刊论文

【摘要】：目的葡萄糖是维持肿瘤细胞存活的重要能量来源,有关研究表明低糖与无糖条件有可能抑制肿瘤细胞生长,成为潜在的肿瘤治疗方式。本研究通过给予不同浓度葡萄糖刺激SKOV3与A2780卵巢癌细胞系,分析其细胞增殖、细胞周期与细胞凋亡的改变,探讨葡萄糖对卵巢癌细胞生长增殖的影响。方法在体外配制含不同葡萄糖浓度(0、2.5、5.5、25.0mmol/L)的培养基,分别模拟无糖、低血糖、正常血糖及高血糖水平的体内环境,观察不同葡萄糖浓度对卵巢癌细胞的增殖、凋亡与细胞周期的影响。结果高糖促进卵巢癌细胞SKOV3和A2780的增殖,干预48h后,增殖幅度约为无糖组的1.841~1.942倍;低糖与无糖条件诱导卵巢癌细胞发生G_1期阻滞,与高糖组相比,SKOV3细胞G_1期比例以(40.699±1.131)%增加至(58.619±2.643)%,A2780细胞以(46.348±2.150)%增加至(54.770±2.475)%;低糖与无糖条件亦诱导细胞凋亡,SKOV3与A2780细胞无糖组中凋亡细胞比例分别为(14.015±0.827)%和(12.930±1.127)%。结论本研究通过探讨葡萄糖对卵巢癌细胞生长与增殖的影响,证明低糖与无糖条件诱导卵巢癌细胞发生G_1期阻滞、细胞凋亡并抑制其生长增殖。若进一步给予相应葡萄糖抑制剂干预,可能为卵巢癌的临床治疗提供新思路。
[Abstract]:Objective glucose is an important energy source to maintain the survival of tumor cells. In this study, SKOV3 and A2780 ovarian cancer cell lines were stimulated with different concentrations of glucose to analyze the changes of cell proliferation, cell cycle and apoptosis. To investigate the effect of glucose on the growth and proliferation of ovarian cancer cells. Methods the culture medium containing different glucose concentrations was prepared in vitro to simulate the in vivo environment of hypoglycemia, hypoglycemia, normal glucose and hyperglycemia, respectively. The effects of glucose concentration on the proliferation, apoptosis and cell cycle of ovarian cancer cells were observed. Results High glucose promoted the proliferation of ovarian cancer cells SKOV3 and A2780. Low glucose and no glucose induced G1-phase arrest in ovarian cancer cells. Compared with the high glucose group, the proportion of G1 phase of SKOV3 cells increased from 40.699 卤1.131% to 58.619 卤2.643% and 46.348 卤2.150% to 54.770 卤2.475.The proportion of apoptotic cells in SKOV3 and A2780 cells was 14.015 卤0.827% and 12.930 卤1.1270.Conclusion the percentage of apoptotic cells in SKOV3 and A2780 cells was 14.015 卤0.827% and 12.930 卤1.1270.Conclusion the ratio of apoptotic cells in SKOV3 and A2780 cells was 14.015 卤0.827% and 12.930 卤1.1270.Conclusion the proportion of apoptotic cells was 14.015 卤0.827% and 12.930 卤1.127% respectively. To investigate the effect of glucose on the growth and proliferation of ovarian cancer cells. The results showed that hypoglycemic and glucose free conditions induced G1-phase arrest, apoptosis and inhibition of growth and proliferation of ovarian cancer cells. Further intervention with glucose inhibitor may provide a new idea for the clinical treatment of ovarian cancer.
【作者单位】：济南大学·山东省医学科学院医学与生命科学学院;山东大学附属山东省肿瘤医院妇瘤科山东省医学科学院;中国医学科学院肿瘤医院深圳医院妇瘤科;山东大学附属山东省肿瘤医院基础实验室;
【基金】：国家自然科学基金(81372778)
【分类号】：R737.31

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