HIF-1α和Rac1在肝细胞癌中的表达及其意义

发布时间：2018-03-11 17:09

本文选题：原发性肝癌　切入点：缺氧诱导因子-α　出处：《中国现代医学杂志》2017年20期 　论文类型：期刊论文

【摘要】：目的探讨缺氧诱导因子-1α(HIF-1α)和Ras相关的C3肉毒底物1(Racl)在肝细胞癌中的表达及其临床意义。方法选取正常肝组织20例、肝硬化组织42例及肝细胞癌组织58例,应用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶连结法检测组织中HIF-1α和Racl蛋白的表达,分析其表达与临床病理特征的关系,并结合临床资料进行分析。结果 HIF-1α主要为胞核或胞浆着色,Rac1为胞浆着色。HIF-1α蛋白在正常肝组织中的阳性表达率为0.0%(0/20),肝硬化组织为45.2%(19/42),原发性肝癌组织为81.0%(47/58),其中高分化阳性表达率为53.3%,中分化为83.3%,低分化为100.0%。HIF-1α蛋白在原发性肝癌发生、发展中的表达依次升高(P0.05)。且原发性肝癌组织中HIF-1α蛋白表达与淋巴结转移有关。Rac1蛋白表达在正常肝组织中的阳性表达率为0.0%(0/20),癌旁肝硬化组织为40.5%(17/42),原发性肝癌组织为77.6%(45/58),其中高分化阳性表达率为60%,中分化为73%,低分化为94.7%。Rac1蛋白在肝癌发生、发展中的表达依次升高(P0.05)。肝癌组织Rac1蛋白表达与淋巴结转移有关。HIF-1α蛋白的阳性表达率与Rac1蛋白的阳性表达率呈正相关(P0.05)。结论 HIF-1α、Rac1与原发性肝癌癌变及进展有关,联合检测两者蛋白的表达有助于判断肝癌的病变程度及预后。
[Abstract]:Objective to investigate the expression and clinical significance of hypoxia inducible factor-1 伪 (HIF-1 伪) and Ras related C 3 botulinum substrates (C 3) in hepatocellular carcinoma (HCC). Methods 20 cases of normal liver tissue, 42 cases of liver cirrhosis and 58 cases of hepatocellular carcinoma were selected. The expression of HIF-1 伪 and Racl protein in tissues was detected by immunohistochemical streptomyces biotin protein-peroxidase linkage method, and the relationship between the expression and clinicopathological characteristics was analyzed. Results the positive expression rate of HIF-1 伪 in normal liver tissue was 0.00 / 20%, 45.22% and 45.22% in liver cirrhosis, respectively. Results the positive expression rate of HIF-1 伪 in primary liver cancer was 81.0%, and the positive rate of HIF-1 伪 protein in normal liver tissue was 45.22%, 45.22%, and 81.0%, respectively, with a high score of 47.58%, and that of liver cirrhosis tissue was 45.22%, and that of primary liver cancer tissue was 81.0%. The positive expression rate of HIF-1 伪 was 53.3%, the middle differentiation was 83.3%, and the low differentiation rate was 100.0k.HIF-1 伪 protein occurred in primary liver cancer. The positive rate of expression of HIF-1 伪 protein related to lymph node metastasis was 0.00 / 20 in normal liver tissue, 40.5% to 17% / 42% in paracancerous liver cirrhosis and 40.5% / 42% to primary liver cancer tissue. The positive rate of expression of HIF-1 伪 protein and lymph node metastasis was 0.00 / 20% in normal liver tissue, 40.5% / 42% in paracancerous cirrhosis tissue, respectively. 77.6% 45 / 58%, in which the positive expression rate of high differentiation was 60%, the middle differentiation was 73%, and the low differentiation was 94.7 .Rac1 protein occurred in liver cancer. The expression of Rac1 protein in HCC was related to lymph node metastasis. The positive expression rate of HIF-1 伪 protein was positively correlated with the positive rate of Rac1 protein expression. Conclusion the expression of HIF-1 伪 -Rac1 is related to the carcinogenesis and progression of primary liver cancer. Combined detection of the expression of both proteins is helpful to judge the pathological degree and prognosis of HCC.
【作者单位】：武汉理工大学校医院;南华大学附属第二医院;
【分类号】：R735.7

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