长链非编码RNAFENDRR对宣威肺癌XWLC-05细胞增殖、迁移及侵袭的影响

发布时间：2018-03-12 21:30

本文选题：宣威肺腺癌　切入点：长链非编码RNA　出处：《中国肿瘤生物治疗杂志》2017年07期 　论文类型：期刊论文

【摘要】：目的:检测非编码RNA FENDRR长链在宣威肺腺癌XWLC-05株细胞与非小细胞肺癌(NSCLC)A549株细胞中的表达,及其对细胞增殖、迁移及侵袭的影响。方法:实时荧光定量PCR检测FENDRR基因在XWLC-05与A549细胞中的表达;构建过表达p EX-3-FENDRR质粒并转染XWLC-05细胞,慢病毒干扰LV3-FENDRR载体转染A549细胞,实时荧光定量PCR检测转染后XWLC-05和A549细胞内FENDRR的表达;MTS法检测细胞增殖;Transwell小室法检测细胞迁移及侵袭能力的变化。结果:XWLC-05细胞中FENDRR基因表达明显较A549细胞低,成功提高转染p EX-3-FENDRR的XWLC-05细胞和降低转染LV3-FENDRR质粒的A549细胞中FENDRR m RNA水平。与对照组相比,过表达FENDRR可明显抑制XWLC-05细胞增殖[(1.23±0.13)vs(1.46±0.25),P0.05]并降低其迁移、侵袭能力(P0.05);干扰FENDRR表达,能够促进A549细胞增殖[(0.85±0.02)vs(0.77±0.08),P0.05]、迁移与侵袭能力(P0.05)。结论:lnc RNA FENDRR表达下调与宣威肺腺癌的发生发展相关,其在肿瘤恶性化过程中抑制肿瘤细胞的增殖与转移。
[Abstract]:Objective: to detect the expression of non-coding RNA FENDRR long chain in Xuanwei lung adenocarcinoma XWLC-05 cell line and non-small cell lung cancer cell line A549, and to investigate its effect on cell proliferation. Methods: Real-time fluorescence quantitative PCR was used to detect the expression of FENDRR gene in XWLC-05 and A549 cells, and overexpression of p EX-3-FENDRR plasmid was constructed and transfected into XWLC-05 cells, and lentivirus interfered with LV3-FENDRR vector to transfect A549 cells. Real-time fluorescence quantitative PCR was used to detect the expression of FENDRR in XWLC-05 and A549 cells after transfection. The cell proliferation and transwell chamber assay were used to detect the changes of cell migration and invasion ability. Results the expression of FENDRR gene was significantly lower in WXWLC-05 cells than in A549 cells. Compared with the control group, overexpression of FENDRR could significantly inhibit the proliferation of XWLC-05 cells [1.23 卤0.13 vs 1.46 卤0.25 p0.05] and decrease its migration and invasion ability, and interfere with the expression of FENDRR. It can promote the proliferation of A549 cell line [0.85 卤0.02VSD 0.77 卤0.08P05], and the ability of migration and invasion is P0.05.Conclusion the down-regulation of RNA FENDRR expression is related to the occurrence and development of Xuan Wei lung adenocarcinoma, which inhibits the proliferation and metastasis of tumor cells during the malignant process of tumor.
【作者单位】：昆明医科大学第一附属医院检验科;昆明医科大学第二附属医院检验科;昆明医科大学第一附属医院急诊外科;
【基金】：国家自然科学基金资助项目(No.81460064,No.81460325) 云南省科技厅昆医联合专项应用基础研究基金资助项目(No.2015FB047) 云南省领军人才计划资助项目(L-201202)~~
【分类号】：R734.2

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