NRP-1单抗联合节律化疗对裸鼠胃癌移植瘤生长的抑制

发布时间：2018-03-14 15:55

本文选题：胃癌　切入点：裸鼠移植瘤　出处：《中国肿瘤生物治疗杂志》2017年12期 　论文类型：期刊论文

【摘要】：目的:探讨NRP-1单抗联合多西他赛节律化疗对胃癌裸鼠移植瘤的抗肿瘤疗效。方法:BALB/c裸鼠皮下接种胃癌BGC-823细胞制备移植瘤模型,将荷瘤裸鼠以数字随机表法随机分为对照组、NRP-1单抗组、节律化疗组(MCT)、联合组(NRP-1mAb+MCT),每组6只。除对照组,其余各组于造模第8天开始分别给予相应治疗,给药2周,观察裸鼠一般状况,隔天测量裸鼠体重及肿瘤体积。裸鼠处死后称瘤质量,H-E染色观察瘤组织形态,免疫组化检测裸鼠瘤组织中NRP-1蛋白、VEGF、MVD表达。结果:联合组移植瘤的体积和质量显著低于其他各组[(0.394±0.128)vs(0.748±0.152)、(0.867±0.361)、(1.247±0.494)g;(0.613±0.223)vs(0.866±0.115)、(1.098±0.343)、(1.474±0.644)cm~3。均P0.05],抑瘤率较其他治疗组差异有统计学意义(P0.05)。对照组癌组织细胞生长良好,血管丰富,给药组癌组织出现不同程度的片状坏死,血管成分减少。免疫组化染色显示,对照组NRP-1表达明显高于治疗各组(P0.05),联合组的NRP-1、VEGF、MVD表达均显著低于其余各组(P0.05)。结论:NRP-1单抗联合多西他赛节律化疗可能通过下调NRP-1表达而显著抑制BGC-823胃癌移植瘤的生长及血管生成。
[Abstract]:Objective: to investigate the antitumor effect of NRP-1 monoclonal antibody combined with docetaxel rhythm chemotherapy on transplanted tumor of gastric carcinoma in nude mice. The nude mice were randomly divided into control group (n = 6), rhythm chemotherapy group (n = 6) and combined group (n = 6) with NRP-1mAb MCTA. The general status of nude mice was observed. The body weight and tumor volume of nude mice were measured the next day. Results: the volume and quality of transplanted tumors in the combined group were significantly lower than those in the other groups [0.394 卤0.128 vs 0.748 卤0.152 卤0.867 卤0.361 卤0.494]. The tumor growth rate in the control group was better than that in the control group (P 0.05), and the tumor growth rate of the combined group was significantly higher than that of the other treatment groups (P 0.05), and the tumor growth rate of the combined group was significantly higher than that of the other treatment groups (P 0.05), and the tumor growth rate of the control group was better than that of the other treatment groups (P 0.05), and the tumor growth rate of the combined group was significantly lower than that of the other treatment groups (P 0.05), and the tumor growth rate of the combined group was significantly lower than that of the other treatment groups (P 0.05), and the tumor growth rate of the control group was better than that of the other treatment groups (P 0.05). Blood vessels were abundant, tumor tissues in the administration group showed different degree of flake necrosis and decreased vascular composition. Immunohistochemical staining showed that, The expression of NRP-1 in the control group was significantly higher than that in the treatment group (P 0.05), and the expression of NRP-1VEGFN in the combined group was significantly lower than that in the other groups (P 0.05). Conclusion the growth and angiogenesis of the transplanted tumor of BGC-823 gastric carcinoma may be significantly inhibited by down-regulation of NRP-1 expression in the control group with docetaxel rhythm chemotherapy.
【作者单位】：厦门大学附属成功医院/中国人民解放军第174医院肿瘤治疗中心;厦门大学医学院抗癌研究中心;
【基金】：厦门市科技计划创新项目资助(No.3502z20134026,No.3502z20144034)~~
【分类号】：R735.2

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