橙皮苷对TGF-β1诱导的A549非小细胞肺癌细胞上皮间质转化的影响

发布时间：2018-03-15 06:24

本文选题：橙皮苷　切入点：非小细胞肺癌细胞　出处：《中国医院药学杂志》2017年08期 　论文类型：期刊论文

【摘要】：目的:探讨橙皮苷对转化生长因子-β1(transforming growth factor-β1,TGF-β1)诱导的人非小细胞肺癌细胞上皮-间质转化的影响。方法:以A549人非小细胞肺癌细胞系作为研究对象。用MTT法,分别检测橙皮苷对正常培养条件或TGF-β1刺激下细胞增殖的影响。应用2种方法评估橙皮苷对TGF-β1刺激条件下A549细胞的上皮间质转化:圆形度值定量评估细胞的形态变化;双抗体夹心酶联免疫吸附法(enzyme linked-immuno-sorbent assay,ELISA)检测上皮细胞钙黏蛋白(E-cadherin,E-cad)、ɑ-平滑肌肌动蛋白(ɑ-smooth muscle actin,ɑ-SMA)、基质金属蛋白酶抑制因子-1(tissue inhibitors of metalloproteinases-1,TIMP-1)和基质金属蛋白酶-9(metallopreoteinases-9,MMP-9)水平。结果:0~40μmol·L-1的橙皮苷对正常培养条件或TGF-β1刺激后A549细胞的增殖没有显著影响。5 ng·mL-1 TGF-β1能够诱导A549细胞的上皮间质转化:由上皮特征的铺路石状的细胞形态转化为梭形形态,圆形度值减少;E-cad分泌量减少和ɑ-SMA合成量增加。另外,MMP-9分泌量增加,MMP-9/TIMP-1比值增加。在细胞出现形态改变后,应用橙皮苷(20μmol·L-1或40μmol·L-1)能够降低ɑ-SMA、MMP-9水平和MMP-9/TIMP-1比值,增加E-cad水平,对细胞的圆形度值没有影响。结论:橙皮苷减轻TGF-β1诱导的非小细胞肺癌细胞的上皮间质转化程度,对非小细胞肺癌的转移和扩散有一定的抑制作用。
[Abstract]:Objective: to investigate the effect of hesperidin on the epithelial-interstitial transformation of human non-small cell lung cancer cells induced by transforming growth factor- 尾 1 (TGF- 尾 1). Methods: A549 human non-small cell lung cancer cell line was used as the study object. The effects of hesperidin on the proliferation of A549 cells stimulated by TGF- 尾 1 or normal culture were determined. The epithelial mesenchymal transformation of A549 cells stimulated by hesperidin was evaluated by two methods: the roundness value was used to quantitatively evaluate the morphological changes of A549 cells. Enzyme linked immunosorbent assay (Elisa) was used to detect E-cadherin, smooth muscle actin, matrix metalloproteinase-1 inhibitors of metalloproteinases-1 and metalloproteinase-9 metallopreoteines-9MMP-9 in epithelial cells. Results the level of hesperidin was 40 渭 mol 路L-1. The proliferation of A549 cells stimulated by normal culture condition or TGF- 尾 1 had no significant effect on the proliferation of A549 cells. 5ng 路mL-1 TGF- 尾 1 could induce the epithelial interstitial transformation of A549 cells. In addition, the secretion of MMP-9 was increased and the ratio of MMP-9 / TIMP-1 was increased. After the morphological change of cells, the level of MMP-9 and the ratio of MMP-9/TIMP-1 were decreased and the level of E-cad was increased after the application of hesperidin 20 渭 mol 路L -1 or 40 渭 mol 路L -1). Conclusion: hesperidin can reduce the degree of epithelial mesenchymal transformation induced by TGF- 尾 1 and inhibit the metastasis and diffusion of NSCLC.
【作者单位】：河南中医药大学;呼吸疾病诊疗与新药研发河南省协同创新中心;河南中医药大学第一附属医院;河南中医药研究院附属医院;
【基金】：国家自然科学基金项目(编号:81473650) 河南省高等学校重点科研项目计划(编号:16A360002)
【分类号】：R734.2

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