Rad9、Rad1、Hus1基因在结直肠癌中的表达及意义

发布时间：2018-03-16 21:35

  本文选题：结直肠癌　切入点：荧光定量PCR　出处：《川北医学院》2017年硕士论文　论文类型：学位论文

【摘要】：目的:检测Rad9、Rad1、Hus1基因的mRNA在结直肠癌组织及癌旁正常组织中的表达水平,并结合肿瘤的病理分化程度、分期、淋巴结转移等临床病理资料,探讨其表达水平是否与结肠癌发病、侵袭及转移有关,为其能否成为结直肠癌重要的生物学标志物提供参考,为直肠癌的早期诊断、早期治疗、预后判断提供依据。方法:收集35例结直肠癌组织及癌旁正常组织,采用荧光定量PCR技术检测组织中Rad9、Rad1、Hus1基因的mRNA表达水平,分析其表达水平在癌组织及癌旁正常组织中有无差异,并进一步分析其表达差异与肿瘤的TNM分期、病理分化程度、部位、性别、年龄等临床病理资料是否有关。研究结果采用Spss17.0软件进行统计学分析,P0.05为差异有统计学意义。结果:1.25例(71.43%)结直肠癌组织中Rad9 m RNA表达量较癌旁正常组织降低,结直肠癌组织中Rad9 mRNA的表达量(2.98±5.14)低于癌旁正常组织(7.11±16.82),差异有统计学意义(Z=-2.064,P=0.039)。2.Rad9 mRNA在低分化肿瘤中有13例(92.85%)低表达,在中分化肿瘤中有9例(64.29%)低表达,在高分化肿瘤中有3例(42.86%)低表达,差异有统计学意义(P=0.033)。3.Rad9mRNA在有淋巴结转移的组织中有16例(88.89%)低表达,无淋巴结转移的组织中有9例(52.94%)低表达,差异有统计学意义(P=0.027)。4.Rad9 mRNA在结直肠癌TNM分期Ⅰ期、Ⅱ期、Ⅲ期、Ⅳ期中分别有1例(25.00%)、8例(61.54%)、10例(83.33%)、6例(100%)低表达,差异有统计学意义(P=0.042)。5.Rad9mRNA在不同年龄、性别和部位的表达差异无统计学意义(P0.05)。6.16例(45.71%)结直肠癌组织中Rad1mRNA的表达量较正常组织降低,结直肠癌组织中的表达量(2.01±4.62)与正常组织中的表达量(2.61±6.49)差异无统计学意义(Z=-0.402,P=0.712)。7.14例(40.00%)结直肠癌组织中Hus1 mRNA表达量较正常组织降低,结直肠癌中的表达量(4.04±7.51)与正常组织中的表达量(3.36±8.30)差异无统计学意义(Z=-0.292,P=0.784)。结论:1.结直肠癌肿瘤组织中Rad9 mRNA的表达量较癌旁正常组织降低,提示Rad9基因可能和结直肠癌的发病有关。2.结直肠癌组织中Rad9 mRNA的低表达率随淋巴结转移、病理分化程度降低及肿瘤的分期级别增高而升高,提示Rad9基因可能与结直肠癌的侵袭及转移有关。3.目前尚不能认为Rad9基因的表达水平与年龄、性别及肿瘤部位有关。4.目前尚不能认为Rad1、Hus1基因与结直癌发病有关。
[Abstract]:Objective: to detect the expression level of Rad9- Rad1hHus1 gene in colorectal cancer and adjacent normal tissues, and to explore whether the expression level of Rad9- Rad1hHus1 gene is associated with the incidence of colon cancer, in combination with the clinical pathological data, such as pathological differentiation, staging, lymph node metastasis, and so on. Invasion and metastasis can be used as an important biomarker for colorectal cancer. Methods: 35 cases of colorectal cancer tissues and adjacent normal tissues were collected for early diagnosis, early treatment and prognosis judgment. Fluorescence quantitative PCR technique was used to detect the expression of Rad9hRd1hHus1 gene in tissues, and to analyze whether there were differences in the expression levels of Rad9hRd1hHus1 gene in cancer tissues and adjacent normal tissues, and to further analyze the difference between the expression levels and the TNM stage, pathological differentiation degree and location of the tumor. Sex, age and other clinicopathological data were related. The results showed that the expression of Rad9 m RNA in colorectal cancer tissues was significantly lower than that in adjacent normal tissues by using Spss17.0 software (P0.05). The expression of Rad9 mRNA in colorectal cancer tissues was 2.98 卤5.14) lower than that in adjacent normal tissues (7.11 卤16.82). The difference was statistically significant. There were 13 cases of poorly differentiated tumors with low expression of Rad9 mRNA and 9 cases with moderately differentiated tumors (64.29%). The low expression of Rad9 mRNA was found in 16 cases with lymph node metastasis (16 cases with lymph node metastasis) and 9 cases without lymph node metastasis (9 cases with lymph node metastasis). There was a significant difference in the expression of Rad9 mRNA in TNM stages 鈪，

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