格列本脲对U-251细胞增殖迁移的影响

发布时间：2018-03-16 21:46

本文选题：格列本脲　切入点：钾离子　出处：《重庆医科大学学报》2017年01期 　论文类型：期刊论文

【摘要】：目的:探讨格列本脲对U-251细胞增殖迁移的影响。方法:使用浓度为12.5~1 600μmol/L的格列本脲(Glib)处理人胶质瘤细胞U-251,应用细胞增殖毒性检测试剂盒测定细胞增殖率并进行药物浓度筛选,划痕实验和Transwell法检测各实验组(使用浓度为100μmol/L、300μmol/L、500μmol/L的Glib处理细胞)及对照组(未加Glib)细胞迁移与侵袭情况。结果:CCK8法检测显示,在100~800μmol/L范围内,Glib可抑制U-251细胞增殖,并呈浓度依赖性,其48 h的半数抑制浓度(IC50)为400μmol/L(F=150.30,P=0.000);划痕实验显示Glib实验组细胞在24 h及48 h迁移宽度明显小于对照组,且随着药物浓度的增加迁移宽度比率降低(F=130.60,P=0.000);Transwell结果显示,Glib抑制细胞迁移能力与划痕试验结果一致,Transwell显示实验组迁移细胞数比对照组明显减少(F=86.68,P=0.000),实验组侵袭细胞数量比对照组明显下降(F=55.30,P=0.000),随着Glib浓度的增加,抑制迁移和侵袭能力逐渐增强,具有浓度依赖性。结论:格列本脲能够抑制U-251细胞增殖迁移过程。
[Abstract]:Objective: to investigate the effect of glibenclamide on the proliferation and migration of U-251 cells. Methods: glibenclamide at a concentration of 12.5 渭 mol/L was used to treat human glioma cells U-251, and cell proliferation rate was determined by cytotoxicity test kit and drug concentration was screened. Scratch test and Transwell assay were used to detect the migration and invasion of U-251 cells in each experimental group (treated with 100 渭 mol / L ~ (300 渭 mol / L) Glib) and in the control group (without Glib). Results: glib could inhibit the proliferation of U-251 cells in a concentration-dependent manner in the range of 100 渭 mol / L ~ (#number0#) 渭 mol/L. IC50 at 48 h was 400 渭 mol 路L ~ (-1) F _ (150.30) P _ (0.000), and scratch test showed that the migration width of Glib experimental group at 24 h and 48 h was significantly smaller than that of control group. With the increase of drug concentration, the ratio of migration width decreased with the increase of drug concentration. The results of Transwell showed that glib inhibited the migration of the cells. Transwell showed that the number of migration cells in the experimental group was significantly lower than that in the control group. The number of invasive cells in the experimental group was significantly lower than that in the control group. The number of invasive cells in the experimental group was significantly lower than that in the control group. Compared with the control group, the concentration of Glib increased with the increase of Glib concentration. Conclusion: glibenclamide can inhibit the proliferation and migration of U-251 cells.
【作者单位】：重庆医科大学基础医学院人体解剖教研室;
【分类号】：R739.41

【相似文献】