8-氯腺苷调节RNA编辑酶ADAR1对乳腺癌SK-BR-3细胞增殖和迁移的影响

发布时间：2018-03-18 05:35

  本文选题：-氯腺苷　切入点：乳腺癌　出处：《重庆医科大学学报》2017年11期 　论文类型：期刊论文

【摘要】：目的:研究8-氯腺苷(8-chloro-adenosine,8-Cl-Ado)对乳腺癌SK-BR-3细胞增殖和迁移的影响,以及与RNA编辑酶1(adenosine deaminases acting on RNA 1,ADAR1)表达的相关性。方法:蛋白印迹实验检测乳腺癌组织中ADAR1的蛋白表达量以及8-Cl-Ado(10μmol/L)处理乳腺癌SK-BR-3细胞不同时间ADAR1蛋白表达量的变化;MTT法和形态学观察检测不加药组和8-Cl-Ado(10μmol/L)加药组对SK-BR-3细胞增殖的影响;MTT法和伤口愈合实验检测SK-BR-3细胞过表达ADAR1质粒(空白对照组、空载组、ADAR1-P110组、ADAR1-P150组)对8-Cl-Ado作用SK-BR-3细胞增殖及迁移的影响。结果:乳腺癌癌组织中ADAR1-P110和ADAR1-P150蛋白表达量均明显高于癌旁组织(t=9.871,P=0.000;t=7.169,P=0.000);8-Cl-Ado作用SK-BR-3细胞48 h后ADAR1-P110和ADAR1-P150蛋白表达量均明显降低(t=-8.838,P=0.013;t=-19.866,P=0.003);8-Cl-Ado作用SK-BR-3细胞ADAR1-P110组和ADAR1-P150组48 h后增殖抑制率均明显低于空白组(P均为0.000);8-Cl-Ado作用SK-BR-3细胞ADAR1-P110组和ADAR1-P150组48 h后的迁移率均明显高于空白组(P均为0.000)。结论:8-Cl-Ado能明显抑制SK-BR-3细胞增殖和迁移,其作用机制可能与ADAR1的表达下调有关。
[Abstract]:Objective: to study the effects of 8-chloro-adenosine 8-chloro-adenosine 8-Cl-Ado) on the proliferation and migration of breast cancer SK-BR-3 cells. Methods: Western blot assay was used to detect the expression of ADAR1 protein in breast cancer tissues and the changes of ADAR1 protein expression in breast cancer SK-BR-3 cells treated with 8-Cl-Adol 10 渭 mol / L at different times. And morphological observation to detect the effect of adding 8-Cl-Adol 10 渭 mol / L on the proliferation of SK-BR-3 cells and the wound healing test to detect the overexpression of ADAR1 plasmid in SK-BR-3 cells (blank control group). The effect of ADAR1-P110 group on proliferation and migration of SK-BR-3 cells induced by 8-Cl-Ado. Results: the expression of ADAR1-P110 and ADAR1-P150 protein in breast cancer tissues was significantly higher than that in adjacent tissues of SK-BR-3 cells, ADAR1-P110 and ADAR1-P150 protein expression were significantly higher than those in the adjacent tissues of SK-BR-3 cells treated with 8-Cl-Ado for 48 h after treatment with ADAR1-P110 group (ADAR1-P110). The inhibition rate of proliferation in ADAR1-P110 group and ADAR1-P150 group after 48 h was significantly lower than that in ADAR1-P110 group and ADAR1-P150 group (P < 0. 000). The migration rate of SK-BR-3 cells in ADAR1-P110 group and ADAR1-P150 group was significantly higher than that in control group (P = 0. 000). Conclusion the inhibition rate of proliferation in ADAR1-P110 group and ADAR1-P150 group is significantly lower than that in ADAR1-P110 group and ADAR1-P150 group after 48 h. Conclusion: 8-Cl-Ado can significantly inhibit the proliferation of SK-BR-3 cells in ADAR1-P110 group and ADAR1-P150 group after 48 hours of treatment. Conclusion: 8-Cl-Ado can significantly inhibit the proliferation of SK-BR-3 cells in ADAR1-P110 group and ADAR1-P150 group after 48 h. Conclusion:% 8-Cl-Ado can significantly inhibit the proliferation of SK-BR-3 cells. Proliferation and migration of SK-BR-3 cells, Its mechanism may be related to the down-regulation of ADAR1 expression.
【作者单位】： 重庆医科大学分子医学与肿瘤研究中心;
【分类号】：R737.9
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本文编号：1628262