miR-630在胃癌组织中的表达变化及对胃癌细胞SGC-7901增殖、侵袭能力的影响

发布时间：2018-03-18 18:55

  本文选题：胃癌　切入点：胃癌细胞　出处：《山东医药》2017年15期 　论文类型：期刊论文

【摘要】：目的观察miR-630在胃癌组织中的表达变化,及miR-630对胃癌细胞株SGC-7901增殖、侵袭能力的影响。方法收集131例胃癌患者的胃癌组织及癌旁正常胃组织,采用实时荧光定量PCR法检测miR-630。培养SGC-7901细胞,分为实验组、空载组和对照组,实验组转染miR-630,空载组转染空质粒,对照组仅加入PBS;分别于培养12、24、48、72 h后采用MTT法检测细胞增殖能力;培养24 h后以Transwell小室检测细胞侵袭能力;培养24 h后,采用Western blotting法检测细胞中的p27、MMP-2、MMP-9蛋白。结果胃癌组织、癌旁组织中miR-630的相对表达量分别为1.93±0.41、2.74±0.48,胃癌组织中miR-630相对表达量低于癌旁组织(P均0.05)。肿瘤直径≥5 cm者胃癌组织中miR-630相对表达量低于直径5 cm者,有淋巴结转移者低于无淋巴结转移者,浸润深度为T2~T4级者低于T1级者,Ⅲ~Ⅳ期者低于Ⅰ~Ⅱ期者(P均0.05)。转染24、48、72 h后,实验组细胞增殖能力均低于转染同时点的空载组和对照组(P均0.01)。实验组、空载组、对照组穿膜细胞数分别为15.4±2.7、24.3±23.1、29.7±19.4,实验组穿膜细胞数少于对照组和空载组(P均0.01)。实验组细胞中p27蛋白相对表达量高于空载组和对照组,MMP-2、MMP-9蛋白相对表达量低于空载组和对照组(P均0.01)。结论胃癌组织中miR-630表达减少,mir-630低表达可能与胃癌的发生发展有关;miR-630有抑制胃癌细胞增殖、侵袭的作用,机制可能与p27蛋白表达上调及MMP-2、MMP-9蛋白表达下调有关。
[Abstract]:Objective to observe the expression of miR-630 in gastric carcinoma and the effect of miR-630 on the proliferation and invasion of gastric cancer cell line SGC-7901. Methods gastric cancer tissues and adjacent normal gastric tissues were collected from 131 patients with gastric cancer. The cultured SGC-7901 cells were divided into experimental group, no-load group and control group. The experimental group was transfected with miR-630, the no-load group was transfected with empty plasmid, and the control group was only added into the control group. After 24 h culture, the invasion ability of the cells was detected by Transwell chamber, and the p27 MMP 2 MMP-9 protein was detected by Western blotting assay after 24 h culture. The relative expression of miR-630 in paracancerous tissues was 1.93 卤0.41t2.74 卤0.48.The relative expression of miR-630 in gastric cancer was lower than that in paracancerous tissues (P < 0.05). The relative expression of miR-630 in gastric cancer tissues with tumor diameter 鈮，

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