喉乳头状瘤患儿人乳头状瘤病毒感染的免疫功能变化研究

发布时间：2018-03-18 18:21

本文选题：人乳头状瘤病毒　切入点：喉乳头状瘤　出处：《中华医院感染学杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的观察喉乳头状瘤患儿感染人乳头状瘤病毒(HPV)的免疫功能变化,探讨HPV感染与免疫功能变化的关系。方法选择浙江省舟山医院和浙江大学附属一院2011年1月-2015年12月52例喉乳头状瘤患儿为观察组及52例健康儿童为对照组,喉乳头状瘤患儿组织HPV及分型采用荧光定量聚合酶链反应法检测,测定血清IgM、IgA和IgG含量及血清IL-10、IL-6、IL-4、TNF-α和IFN-γ水平,测定外周血CD4+、CD3+、CD8+含量。结果 52例患儿中HPV11感染32例,HPV6感染20例;观察组发病时间和HPV病毒载量呈负相关(r=-0.341,P=0.043),两组患儿血清IgM、IgA和IgG含量比较差异无统计学意义;观察组患儿CD4+(29.87±7.83)%和CD3+(59.89±9.78)%均低于对照组(36.41±7.67)%、(67.23±8.24)%,差异有统计学意义(P0.05);观察组患儿血清IL-6[3.07(2.45~8.34)pg/ml]、IL-4[(2.59±1.43)pg/ml]、TNF-α[2.68(1.47~4.37)pg/ml]和IFN-γ[(4.76±2.68)pg/ml]水平均高于对照组[2.12(1.56~2.67)pg/ml、(1.78±0.73)pg/ml、[1.39(1.06~1.98)pg/ml]、(2.41±0.79)pg/ml],差异有统计学意义(P0.05)。结论 HPV11型和HPV6型为儿童喉乳头状瘤HPV感染类型,发病时间和HPV病毒载量呈负相关,喉乳头状瘤HPV感染表现为免疫抑制,免疫功能变化主要以细胞免疫改变,细胞因子水平升高。
[Abstract]:Objective to observe the changes of immune function in children with laryngeal papilloma infected with human papillomavirus (HPV). Methods 52 cases of laryngeal papilloma from January 2011 to December 2015 in Zhoushan Hospital of Zhejiang Province and the first affiliated Hospital of Zhejiang University were selected as observation group and 52 healthy children as control group. The tissue HPV and classification of children with laryngeal papilloma were detected by fluorescence quantitative polymerase chain reaction (FQ-PCR). The levels of IgA and IgG in serum, the levels of IL-10, IL-6, IL-4, TNF- 伪 and IFN- 纬 in serum, and the levels of CD4 CD3 and CD8 in peripheral blood were measured. Results among the 52 cases, 32 cases were infected with HPV11 and 20 cases were infected with HPV6. In the observation group, there was a negative correlation between the time of onset and the load of HPV virus. There was no significant difference in serum IgA and IgG levels between the two groups. The levels of CD4 in observation group (29.87 卤7.83%) and CD3 group (59.89 卤9.78%) were significantly lower than those in control group (36.41 卤7.67 卤67.23 卤8.24), and the levels of serum IL-6 in observation group [3.07 卤2.45 卤8.34 PG / ml] IL-4 [2.59 卤1.43 g / ml] TNF- 伪 [2.681.471.474.37g / ml] and IFN- 纬 [4.76 卤2.68pg / ml] were significantly higher than those in control group [2.121.561.562.67pggml 1.78 卤0.73pgml-1 / g / ml] 2.396981g / ml] 2.79g / ml, respectively. There was a negative correlation between the time of onset and the load of HPV virus. The HPV infection of laryngeal papilloma showed immunosuppression. The main changes of immune function were cellular immunity and cytokine level increased.
【作者单位】：浙江省舟山医院耳鼻咽喉科;浙江省舟山医院检验科;浙江大学附属第一医院耳鼻咽喉头颈外科;
【基金】：浙江省医药卫生一般研究计划(2014KYB040)
【分类号】：R739.65

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