整合组学数据分析乳腺癌细胞与外周免疫系统的交互作用

发布时间：2018-03-19 16:39

本文选题：乳腺癌　切入点：外周血　出处：《肿瘤防治研究》2017年04期 　论文类型：期刊论文

【摘要】：目的探讨乳腺癌上皮细胞与外周血之间的交互作用。方法从GEO数据库中获取乳腺癌上皮细胞及外周血单个核细胞(PBMCs)表达谱。GEO2R在线分析工具筛选差异表达基因,利用Uni Prot和STRING数据库对差异表达基因蛋白产物进行亚细胞定位,筛选乳腺癌细胞分泌蛋白及与其相互作用的PBMCs膜蛋白。Cytoscape软件构建互作网络。使用DAVID在线工具基因功能注释和KEGG通路富集方法对互作分子进行分析。结果共筛选到101对相互作用分子,基因功能主要涉及细胞黏附、细胞迁移、白细胞激活、免疫反应等生物学过程,参与的信号通路主要有细胞外基质—受体相互作用,细胞因子—细胞因子受体相互作用,趋化因子信号通路等。其中FN1、ITGBI、FLT1、COMP、CXCL12为关键蛋白。结论利用生物信息学方法,整合组学数据,能够有效获取信息,为乳腺癌的治疗及预后监测的研究开辟新思路。
[Abstract]:Objective to investigate the interaction between breast cancer epithelial cells and peripheral blood. Methods the expression profiles of breast cancer epithelial cells and peripheral blood mononuclear cells (PBMCs) were obtained from GEO database. GEO2R online analysis tool was used to screen differentially expressed genes. Subcellular localization of differentially expressed gene protein products was performed by using Uni Prot and STRING databases. To screen the secreting proteins of breast cancer cells and their interaction with PBMCs membrane protein. Cytoscape software was used to construct the interaction network. The interaction molecules were analyzed by DAVID online tool gene function annotation and KEGG pathway enrichment method. 101 pairs of interacting molecules, Gene function is mainly involved in biological processes such as cell adhesion, cell migration, leukocyte activation, immune response and so on. The signal pathways involved include extracellular matrix receptor interaction and cytokine cytokine receptor interaction. CXCL12 is a key protein in chemokine signaling pathway. Conclusion Bioinformatics and data integration can effectively obtain information and open up a new way for the study of breast cancer treatment and prognosis monitoring. [WT5 "HZ] [WT5" HZ] [WT5 "BZ] [WT5" HZ] [WT5BZ] CXCL12 is a key protein in chemokine signaling pathway.
【作者单位】：四川省肿瘤医院研究所医务部;成都医学院生物医学系;
【基金】：国家自然科学基金(81201702) 四川省卫计委科研项目(130295)
【分类号】：R737.9

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