麝香对人胃癌细胞株裸鼠移植瘤VEGF、Bcl-2、Bax及瘤体的影响

发布时间：2018-03-20 12:09

本文选题：麝香　切入点：血管内皮生长因子　出处：《青海大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:探讨麝香对人胃癌细胞株裸鼠移植瘤VEGF、Bcl-2、Bax的表达及瘤体的影响。方法:采用裸鼠皮下注射人胃癌细胞(BGC-823)建立肿瘤模型。14 d行病理切片验证造模是否成功,将造模成功的35只裸鼠,随机分为模型对照组、氟尿嘧啶组、麝香低、中、高剂量组,每组7只。并给予相应的用药处理。分组后每5 d测量并计算每组平均瘤体积。用药21 d后观察各组相关指标的表达水平。结果:用药各组移植瘤均出现不同程度的抑制(P0.05)。用药各组的移植瘤质量明显低于模型组(P0.05)。VEGF平均灰度的表达麝香高剂量组较模型组明显偏低(P0.05);平均面密度表达麝香高剂量组较模型组显著减少(P0.05)。Bcl-2平均灰度的表达麝香高、中剂量组较模型组显著偏低(P0.05);平均面密度表达各用药组与模型对照组比较无明显差异(P0.05)。Bax平均灰度的表达中麝香各组较模型组显著升高(P0.05);平均面密度表达中麝香各用药组较模型组显著增加(P0.05)。结论:麝香对胃癌裸鼠异位移植瘤具有抑制肿瘤生长的效果,可以减缓癌细胞的进一步增殖和分化,麝香抑瘤机制可能与下调肿瘤组织血管内皮生长因子及Bcl-2表达活性、上调Bax表达活性相关。
[Abstract]:Objective: To investigate the effect of musk Bcl-2 on human gastric carcinoma cell line VEGF in nude mice, and the tumor body, affecting the expression of Bax. Methods: the subcutaneous injection of human gastric cancer cells (BGC-823).14 D tumor model was established by pathological section to verify the success of modeling, will build 35 nude rat model successfully, randomly divided into the model control group, 5-fluorouracil group, musk, low and high dose group, 7 rats in each group. And give the corresponding drug treatment group. Each of the 5 d were measured in each group. The average tumor volume of the expression level of related indicators were observed after 21 d treatment. Results: the treatment group tumor appeared different degree of inhibition (P0.05). The tumor mass in each medication group was significantly lower than that of model group (P0.05) expression of musk in high dose group the average gray level of.VEGF is higher than that of model group (P0.05); the average low surface density expression musk high dose group with the model group were significantly reduced (P0.05) average grey.Bcl-2 The expression of musk high dose group were significantly lower than those in the model group (P0.05); the average surface density of expression of the treatment group and model control group had no significant difference (P0.05) expression of.Bax in each group the average gray musk than in the model group increased significantly (P0.05); the average surface density of musk in the treatment group significantly compared with the model group increased (P0.05). Conclusion: musk has the effect of inhibiting tumor growth of gastric cancer in nude mice xenograft tumor, cancer cells can slow further proliferation and differentiation, musk tumor suppressor mechanism might be related to the down regulation of vascular endothelial growth factor in tumor tissue and the expression of Bcl-2, up regulate the expression of Bax.

【学位授予单位】：青海大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.2

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