FGF3在大肠癌中的表达及临床意义研究

发布时间：2018-03-20 13:31

本文选题：大肠癌　切入点：FGF3　出处：《河北医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:大肠癌是全世界最常见的高发恶性肿瘤之一,近年来大肠癌的发病率呈现出上升趋势。大肠癌的发病原因复杂并未完全阐明,但多数学者认为大肠癌的发生是由多种因素及多种基因相互作用而逐步发展的结果。目前有大量研究表明,人类多数肿瘤的发生、发展过程均与FGF3成纤维细胞生长因子3基因表达相关,并与肿瘤的恶性分化及预后有关。本实验通过实时荧光定量聚合酶链反应(real-time PCR)检测大肠癌患者癌组织和其对应癌旁组织中FGF3 mRNA的相对表达量水平,通过对实验数据及临床病理资料的分析,研究FGF3 mRNA与大肠癌之间的关系,为深入了解FGF3在大肠癌发病过程中发挥的作用提供理论依据。方法:1收集2015年9月~2016年10月51对来自河北医科大学第一医院普外科大肠癌及对应癌旁组织手术标本。大肠癌组织定义为病理证实的无坏死、穿孔及溃疡的原发大肠癌组织;癌旁组织定义为距离肿瘤边缘5cm以上的病理证实的非癌组织,并且无坏死、穿孔及溃疡的大肠组织。新鲜组织标本离体后迅速置于冷处理后的1.5ml EP管中,并放入-80℃超低温冰箱中保存。51名患者术前从未进行任何方案的化学治疗和放射治疗。2大肠癌组织及癌旁组织中总RNA的提取是严格按照美国Invirotrogen公司Trizol试剂说明书步骤方法进行提取操作。然后按照美国genecopoeia公司反转录试剂盒说明书步骤进行反转录,获得cDNA。3应用real-time PCR方法检测51对大肠癌组织及对应癌旁组织的CT值,以GAPDH为内参,用2-△CT方法计算FGF3mRNA在大肠癌组织、癌旁组织中的相对表达量。比较两组间表达差异,然后再以不同的病理资料进行分组,比较不同组之间癌组织相对表达量的差异。4采用SPSS19.0及GraphPad Prism 5.0统计软件,对实验结果进行分析,计量资料以中位数(四分位数)表示,两组间配对样本采用非参数检验。以P0.05表示差异存在有统计学意义。结果:1FGF3在51例大肠癌组织中相对表达量0.00054(0.00011,0.00154),癌旁组织中表达量0.00306(0.00096,0.01170),可见FGF3在大肠癌组织中的表达明显低于癌旁组织的表达,差异有统计学意义(p0.05,见fig.1)。251位患者平均年龄约为65岁,以年龄分组,其中小于65岁的患者组大肠癌组织中FGF3的相对表达量为0.00087(0.00011,0.00234),年龄等于或大于65岁的患者组大肠癌组织中FGF3的相对表达量为0.00050(0.00011,0.00116)。小于65岁的患者组大肠癌组织中FGF3的相对表达量稍高于大于或等于65岁的患者组,但两组之间无统计学差异(p0.05,见fig.2、table.1)。3以性别分组,51例直肠癌患者分为男性组和女性组,分别为33名男性患者和18名女性患者,男性组大肠癌组织中FGF3的相对表达量为0.00040(0.00011,0.00146),女性组大肠癌组织中FGF3的相对表达量为0.00057(0.00017,0.00246),女性组癌组织中FGF3的相对表达量稍高于男性组,但无统计学差异(p0.05,见fig.3、table.1)。451例直肠癌患者包括22名有淋巴结转移的患者和29名无淋巴结转移的患者,其中有淋巴结转移的患者组大肠癌组织中FGF3的相对表达量为0.00057(0.00011,0.00178),无淋巴结转移的患者组大肠癌组织中FGF3的相对表达量为0.00040(0.00014,0.00157),有淋巴结转移的患者组大肠癌组织中FGF3的相对表达量稍高于无淋巴结转移的患者组,两组之间无统计学差异(p0.05,见fig.4、table.1)。5按照大肠癌dukes分期为51个大肠癌患者分组,可分为a期,b期,c期,d期。其中a期和b期的大肠癌患者有28人,c期和d期的大肠癌患者有23人。a期和b期的患者直肠癌组织相对表达量为0.00048(0.00017,0.00177),c期和d期的患者直肠癌组织相对表达量为0.00054(0.00011,0.00155),两组之间无统计学差异(p0.05,见fig.5、table.1)。6以大肠癌肿瘤位置分组,其中直肠癌有31人,结肠癌20人,直肠癌组相对表达量为0.00054(0.00011,0.00137),结肠癌组相对表达量为0.00044(0.00013,0.00269),直肠癌组相对表达量稍高于结肠癌组,两组之间无统计学差异(p0.05,见fig.6、table.1)。结论:1FGF3在51例患者癌旁组织中的表达明显高于大肠癌组织,提示FGF3的表达差异可能与大肠癌的发生与发展具有相关性。2 FGF3在51例患者大肠癌组织中表达水平的高低与患者平均年龄、性别、区域淋巴结转移情况、大肠癌的Dukes分期、肿瘤位置并无明显相关性。
[Abstract]:Objective: colorectal cancer is one of the world's most common malignant tumors, the incidence of colorectal cancer showed a rising trend in recent years. The pathogenesis of colorectal cancer complex has not been fully elucidated, but many researchers believe that the incidence of colorectal cancer is caused by multiple factors and multiple gene interactions and the progressive development of the results. There are a large number of studies show that most human tumor occurrence, development process and FGF3 fibroblast growth factor 3 gene expression related to the differentiation and prognosis of malignant tumors. And this experiment by real-time fluorescence quantitative polymerase chain reaction (real-time PCR) expression in tumor tissues of patients with colorectal cancer and corresponding adjacent tissues FGF3 the amount of mRNA level, through the analysis of experimental data and clinical pathological data, study the relationship between FGF3 mRNA and colorectal cancer, for in-depth understanding of FGF3 in the pathogenesis of colorectal cancer in the play Provide a theoretical basis for the effect. Methods: 1 September 2015 ~2016 year in October 51 from the first hospital of Hebei Medical University, Department of general surgery of colorectal cancer and corresponding adjacent tissue specimens. No necrosis of colorectal cancer was defined as pathology, perforation and ulcer of primary colorectal cancer; carcinoma is defined as the distance 5cm above the edge of the tumor pathology confirm the non cancer tissue, and no necrosis, ulcer perforation and colorectal tissue. Fresh tissue specimens rapidly after placed after cold treatment 1.5ml EP tube, extraction and put in the -80 stored at.51 patients in the ultra low temperature freezer before the total RNA of any program of radiotherapy and chemotherapy of colorectal cancer.2 carcinoma is extracted in strict accordance with the American Invirotrogen company Trizol kit method. Then according to the U.S. company genecopoeia reverse transcription Kit Manual steps for reverse transcription, was detected by cDNA.3 real-time PCR of 51 colorectal cancer tissues and corresponding adjacent tissues CT, using GAPDH as internal control, calculation of FGF3mRNA in colorectal cancer tissue by 2- CT method, the relative expression in cancer tissue. The expression differences were compared between the two groups, and then grouping the pathological data of different, different groups were compared between carcinoma relative expression difference of.4 by SPSS19.0 and Prism 5 statistical software GraphPad, the analysis of experimental results, the measurement data to the median (four percentile), between the two groups using paired samples nonparametric test. P0.05 difference was statistically significant results. 1FGF3: in 51 cases of colorectal cancer in the relative expression of 0.00054 (0.00011,0.00154), the expression in cancer tissue was 0.00306 (0.00096,0.01170), expression of FGF3 in colorectal cancer tissues was significantly lower than in adjacent Tissue expression, the difference was statistically significant (P0.05, Fig.1) the average age of.251 patients is about 65 years old, the age group, the relative expression of which is less than the group of patients with FGF3 colorectal cancer at the age of 65 in the amount of 0.00087 (0.00011,0.00234), the age is equal to or greater than 65 years of relative expression of FGF3 in patients with colorectal cancer in the amount of 0.00050 (0.00011,0.00116). The relative expression of FGF3 in patients younger than 65 years old group in colorectal cancer patients is greater than or equal to the amount is slightly higher than that of group 65, but no significant difference between the two groups (P0.05, Fig.2, table.1,.3) by sex group, 51 cases of rectal cancer patients were divided into male group and female group were 33 male patients and 18 female patients, the relative expression of FGF3 in colorectal cancer in male group was 0.00040 (0.00011,0.00146), the relative expression of FGF3 in colorectal carcinoma in the female group was 0.00057 (0.00017,0.00246), female The relative expression of group FGF3 carcinoma is slightly higher than the male group, but no significant difference (P0.05, Fig.3, table.1).451 cases of colorectal cancer patients including 22 patients with lymph node metastasis and 29 without lymph node metastasis, including relative expression in patients with FGF3 colorectal cancer lymph node metastasis in the amount of 0.00057 (0.00011,0.00178), the relative expression of FGF3 in patients without lymph node metastasis group of colorectal cancer in the amount of 0.00040 (0.00014,0.00157), the relative expression of FGF3 in patients with colorectal cancer lymph node metastasis in quantity was slightly higher than that of patients with no lymph node metastasis, there was no significant difference between the two groups (P0.05 see Fig.4,.5, table.1) in colorectal cancer Dukes staging in 51 patients with colorectal cancer group, can be divided into a phase, B phase, C phase, D phase. The A and B of patients with colorectal cancer 28, C and D of patients with colorectal cancer and 23 people.A stage B patients Their relative expression of 0.00048 colorectal cancer (0.00017,0.00177), C and D in patients with rectal cancer relative expression quantity was 0.00054 (0.00011,0.00155), no significant difference between the two groups (P0.05, Fig.5, table.1).6 in colorectal tumor location group, which has 31 human colon cancer rectal cancer, 20 rectal cancer. Group relative expression quantity was 0.00054 (0.00011,0.00137), colon cancer group relative expression was 0.00044 (0.00013,0.00269), the relative expression in rectal cancer group was slightly higher than that of colon cancer group, no significant difference between the two groups (P0.05, Fig.6, table.1). Conclusion: the expression of 1FGF3 in 51 cases of cancer patients was significantly higher than that in the adjacent tissues colorectal cancer, FGF3 expression may have suggested that the expression level of patients with Ping Junnian's age, the relationship between the level of.2 FGF3 in 51 cases of patients with colorectal cancer occurrence and development of colorectal cancer and gender, regional lymph node metastasis There is no significant correlation between the Dukes staging of colorectal cancer and the location of the tumor.

【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.34

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