外周血淋巴细胞亚群检测在肺癌患者临床评估及辅助治疗中的意义

发布时间：2018-03-21 02:29

  本文选题：肺癌　切入点：淋巴细胞　出处：《延安大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:通过观察外周血淋巴细胞亚群在肺癌患者的表达情况,探讨肺癌患者的免疫功能是否存在一定的变化规律,从而更好地评估肺癌患者的病情及预后,以期为其辅助治疗提供更多的参考。方法:选择2015年8月至2016年12月于我院确诊并进行2个周期以上(包括2个周期)化疗的肺癌患者63例,同期体检健康者30例,前者作为观察组,后者作为对照组,借助流式细胞术,测定观察组化疗前及完成第2个周期化疗后的外周血CD3+T淋巴细胞、CD4+T淋巴细胞、CD8+T淋巴细胞和NK细胞的比例,并测定上述指标在对照组的表达情况,比较观察组和对照组之间、观察组中不同病理类型之间、不同临床分期之间以及第一个周期化疗前和第二个周期化疗后上述各指标的差异。所有数据以均数±标准差(?x±s)的形式表示,用SPSS 20.0统计软件(中文版)处理所得数据。以P=0.05作为检验水准判断差异是否具有统计学意义。结果:1.观察组外周血CD3+T淋巴细胞、CD4+T淋巴细胞和NK细胞比例较对照组降低,CD8+T淋巴细胞比例较对照组有所升高。2.不同病理类型的肺癌患者,其外周血CD3+T淋巴细胞、CD4+T淋巴细胞、CD8+T淋巴细胞和NK细胞比例无显著差异。3.不同分期的肺癌患者,分期越晚,其外周血CD3+T淋巴细胞、CD4+T淋巴细胞和NK细胞比例越低,CD8+T淋巴细胞比例越高。4.2个周期化疗后PS评分≤2的肺癌患者,其外周血CD3+T淋巴细胞、CD4+T淋巴细胞和NK细胞比例较化疗前有所升高,CD8+T淋巴细胞比例有所回落;2个周期化疗后PS评分2的肺癌患者,其CD3+T淋巴细胞、CD4+T淋巴细胞、CD8+T淋巴细胞和NK细胞比例较化疗前变化不显著。结论:多数肺癌患者出现不同程度的免疫功能下降,晚期肺癌患者的免疫功能较早期肺癌患者低,不同病理类型肺癌患者的免疫功能无显著差异;规范的化疗可以在一定程度上促进肺癌患者免疫功能的恢复;治疗肺癌时应考虑给予适当的免疫调节治疗作为辅助治疗。
[Abstract]:Objective: to observe the expression of peripheral blood lymphocyte subsets in patients with lung cancer, and to explore whether the immune function of lung cancer patients has a certain change rule, so as to better evaluate the condition and prognosis of lung cancer patients. Methods: from August 2015 to December 2016, 63 patients with lung cancer were diagnosed in our hospital and received 2 cycles of chemotherapy (including 2 cycles). The former served as the observation group and the latter as the control group. Flow cytometry was used to measure the percentage of CD4, CD8 and NK cells in peripheral blood CD3 T lymphocytes before and after the second cycle of chemotherapy in the observation group. The expression of the above indexes in the control group was measured, and the pathological types were compared between the observation group and the control group, and between the observation group and the control group. The differences of the above indexes between different clinical stages, before and after the first cycle chemotherapy and after the second cycle chemotherapy. In the form of x 卤s, SPSS 20.0 statistical software (Chinese version) was used to process the data. The difference was statistically significant using P0. 05 as the test level. Results: 1. The proportion of CD4 T lymphocytes and NK cells in peripheral blood CD3 T lymphocytes in the observation group was higher than that in the control group. The percentage of CD8 T lymphocytes decreased in the radiation group was higher than that in the control group. 2. Patients with different pathological types of lung cancer, There was no significant difference in the proportion of CD8 T lymphocytes and NK cells between CD4 T lymphocytes and NK cells in peripheral blood CD3 T lymphocytes. 3. The later the stage was, the later the stage was in patients with different stages of lung cancer. The lower the ratio of CD4 T lymphocytes to NK cells in peripheral blood CD3 T lymphocytes, the higher the percentage of CD8 T lymphocytes. The patients with PS score 鈮，

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