MG132对卵巢上皮性癌裸鼠移植瘤生长的影响及联合顺铂作用效应的研究

发布时间：2018-03-24 05:37

本文选题：卵巢癌　切入点：蛋白酶体抑制剂　出处：《实用妇产科杂志》2017年02期

【摘要】：目的:探讨蛋白酶体抑制剂MG132应用于卵巢上皮性癌裸鼠移植瘤的新型治疗及联合顺铂协同抗肿瘤的潜在价值和可行性。方法:建立人卵巢癌裸鼠皮下移植瘤模型,20只裸鼠随机平均分为4组,每日腹腔内注射给药1次,共7日。对照组(0.2 ml的0.9%氯化钠液),MG132组(2 mg/kg),顺铂组(1 mg/kg),联合组[MG132(2 mg/kg)+顺铂(1 mg/kg)]。移植瘤模型建立4周后比较各组移植瘤质量抑制率,IHC、FIA、Western blot、RT-PCR检测各组肿瘤促凋亡基因(Caspase3)、自噬基因(Beclin1)的表达情况。结果:1顺铂组、MG132组、联合组对移植瘤的瘤质量抑制率分别为15.38%、53.85%、88.46%,顺铂联合MG132理论相加的瘤质量抑制率为60.95%;2IHC、FIA、Western blot检测:与对照组相比,顺铂组、MG132组、联合组Beclin1、Caspase3阳性表达均明显增强,其中联合组阳性表达更强。3RT-PCR检测:顺铂组、MG132组、联合组的Beclin1、Caspase3 mRNA表达均明显高于对照组(P0.05);且联合组又高于顺铂组、MG132组(P0.05)。结论:MG132对卵巢上皮性癌裸鼠移植瘤生长具有抑制作用,且与顺铂联合治疗卵巢癌具有协同抗肿瘤效应,有望成为治疗顺铂耐药型难治性卵巢癌的一种有效抗肿瘤药物。
[Abstract]:Objective: to investigate the potential value and feasibility of proteasome inhibitor MG132 in the treatment of ovarian epithelial carcinoma in nude mice and the potential value and feasibility of combination of cisplatin and antitumor. Methods: a model of human ovarian cancer xenografts in nude mice was established. Twenty nude mice were randomly divided into 4 groups. Once daily intraperitoneal injection, A total of 7 days. The control group (0. 2 ml of 0.9% sodium chloride solution) and the MG132 group (2 mg / kg), the cisplatin group (1 mg / kg) and the combined group [MG132(2 mg / kg]. Four weeks after the establishment of the transplanted tumor model, the tumor quality inhibition rate of each group was compared by IHC FIA Western blot RT-PCR, and the apoptotic gene Caspase3 was detected by reverse transcription polymerase chain reaction (RT-PCR). Results the MG132 group was treated with cisplatin. The inhibition rate of tumor quality in the combined group was 15.38% and 53.85%, respectively. The inhibition rate of the tumor mass of the combined group was 60.955%. The positive expression of Beclin1 caspase3 in the combined group was significantly higher than that in the control group, compared with the control group, and that in the combination group was significantly higher than that in the control group. The expression of Beclin1 caspase3 mRNA in the combined group was significantly higher than that in the control group (P0.05), and in the combined group was higher than that in the cisplatin group (P 0.05). Conclusion the growth of ovarian epithelial carcinoma xenografts in nude mice was inhibited by WMG132, and the expression of Beclin1 caspase3 mRNA in the combined group was significantly higher than that in the Cisplatin group. The combination of cisplatin and cisplatin in the treatment of ovarian cancer has synergistic antitumor effect and is expected to be an effective antitumor drug for cisplatin resistant refractory ovarian cancer.
【作者单位】：四川大学华西第二医院出生缺陷与相关妇儿疾病教育部重点实验室;
【分类号】：R737.31

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