沉默脂肪甘油三酯脂肪酶基因表达可抑制肝细胞癌细胞的增殖、迁移与侵袭

发布时间：2018-03-29 06:24

本文选题：癌　切入点：肝细胞　出处：《肿瘤》2017年05期

【摘要】：目的:分析肝细胞癌组织及细胞系中脂肪甘油三酯脂肪酶(adipose triglyceride lipase,ATGL)的表达水平,探讨ATGL在肝细胞癌细胞增殖、迁移和侵袭过程中的作用。方法:采用蛋白质印迹法检测57对肝细胞癌组织和相应癌旁正常肝组织,以及12种肝癌细胞系中ATGL的表达水平。将靶向ATGL基因的shRNA重组慢病毒(GV248-shATGL-1、-2和-3)及其阴性对照慢病毒(GV248-shNC)分别感染肝癌Huh7和MHCC-97L细胞系,然后采用实时荧光定量PCR和蛋白质印迹法验证ATGL基因沉默效率。采用CCK-8法和Transwell小室法分别检测ATGL基因沉默对肝癌Huh7和MHCC-97L细胞增殖、迁移和侵袭的影响。结果:57例肝细胞癌患者中,有28例(49%)肝癌组织的ATGL表达水平明显高于其相应癌旁肝组织,16例(28%)肝癌组织的ATGL表达水平低于相应癌旁肝组织,而13例(23%)无明显变化。与阴性对照组相比,沉默ATGL基因表达能够明显抑制肝癌Huh7和MHCC-97L细胞增殖(P值均0.05),同时细胞迁移和侵袭能力均明显降低(P值均0.01)。结论:肝细胞癌组织中ATGL相对高表达,而沉默ATGL基因表达可以抑制肝细胞癌Huh7和MHCC-97L细胞的增殖、迁移和侵袭。
[Abstract]:Objective: to analyze the expression of adipose triglyceride lipase ATGL in hepatocellular carcinoma tissues and cell lines, and to investigate the proliferation of ATGL in hepatocellular carcinoma cells. Methods: Western blot was used to detect 57 pairs of hepatocellular carcinoma tissues and adjacent normal liver tissues. The expression level of ATGL was detected in 12 HCC cell lines. Huh7 and MHCC-97L cells were infected with shRNA recombinant lentivirus (GV248-shATGL-1h-2 and -3) targeting ATGL gene and its negative control lentivirus (GV248-shNCrespectively). Then real-time fluorescence quantitative PCR and Western blotting were used to verify the silencing efficiency of ATGL gene. CCK-8 assay and Transwell chamber assay were used to detect the proliferation of Huh7 and MHCC-97L cells induced by ATGL gene silencing, respectively. Results the expression level of ATGL in HCC tissues was significantly higher than that in adjacent liver tissues (16 cases, 28 cases), and the expression level of ATGL in HCC tissues was significantly lower than that in corresponding adjacent liver tissues, and the expression level of ATGL in HCC tissues was significantly higher than that in adjacent HCC tissues (P < 0.05), and the expression level of ATGL in HCC tissues was significantly higher than that in adjacent HCC tissues (P < 0.05). There was no significant change in 13 cases. Compared with the negative control group, Silencing the expression of ATGL gene could significantly inhibit the proliferation of Huh7 and MHCC-97L cells, and decrease the cell migration and invasion ability. Conclusion: the expression of ATGL is relatively high in HCC. Silencing the expression of ATGL gene can inhibit the proliferation, migration and invasion of Huh7 and MHCC-97L cells.
【作者单位】：上海交通大学医学院附属仁济医院上海市肿瘤研究所癌基因与相关基因国家重点实验室;启东肝癌防治研究所病因研究室;
【基金】：国家自然科学基金资助项目(编号:81372192,81672832)~~
【分类号】：R735.7

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