基于蛋白质相互作用网络对滑膜肉瘤生物学通路探索

发布时间：2018-04-01 04:34

本文选题：滑膜肉瘤　切入点：分子生物学　出处：《中华肿瘤防治杂志》2017年10期

【摘要】：目的滑膜肉瘤(synovial sarcoma,SS)是一种来源不明的软组织恶性肿瘤,临床上较少见。随着分子生物学的不断发展,从分子层面了解滑膜肉瘤并发现其相关生物学通路,以此寻找其治疗靶点成为目前研究的热点。本研究通过建立蛋白质相互作用网络预测滑膜肉瘤的生物学通路,以期发现其相关作用靶点。方法通过在线人类孟德尔遗传数据库筛选出滑膜肉瘤相关基因,采用Cytoscape软件和插件Agilent Literature Search进行文本挖掘并建立滑膜肉瘤的蛋白质相互作用网络;采用插件Clusterviz,发现网络中可能包含的分子复合物;基于DAVID富集分子复合物的生物学通路。结果通过人类孟德尔遗传数据库筛选出滑膜肉瘤相关基因有67个;采用Cytoscape软件挖掘出滑膜肉瘤相关蛋白质相互作用网络包含489个节点(蛋白质)和1 414条边(相互作用关系);Clusterviz插件发现其可能包含6个分子复合物;通过DAVID对这6个分子复合物进行富集,获得其相关生物学通路,以错误发现率(false discovery rate,FDR)1为差异有统计学意义,最终获得分子复合物1有7条通路,分子复合物2有45条通路。分子复合物3、4、5和6的细胞通路FDR值均1,差异无统计学意义。结论分子复合物1主要与p53信号通路及细胞因子调节有关,且与膀胱癌有相关性;分子复合物2涉及通路较多,说明滑膜肉瘤的发生是一个受多种细胞通路、多基因构成的网络协同调控的复杂过程。
[Abstract]:Objective synovial sarcomaosarcoma (SSS) is a kind of soft tissue malignant tumor of unknown origin, which is rarely seen in clinic. With the development of molecular biology, the molecular level of synovial sarcoma is understood and its related biological pathway is discovered. In this study, protein interaction network was established to predict the biological pathway of synovial sarcoma. Methods the genes related to synovial sarcoma were screened by online human Mendelian genetic database, and text mining was carried out by Cytoscape software and plug-in Agilent Literature Search, and protein interaction network of synovial sarcoma was established. Molecular complexes may be found in the network and biological pathways based on DAVID enriched molecular complexes. Results 67 genes related to synovial sarcoma were screened by human Mendelian genetic database. The synovial sarcoma-associated protein interaction network was found to contain 489 nodes (proteins) and 1,414 edges by Cytoscape software. The six molecular complexes were enriched by DAVID and their biological pathways were obtained. The difference of false discovery rateFDR1 was statistically significant, and the molecular complex 1 had 7 pathways. There were 45 pathways in molecular complex 2 and 1 in cell pathway of molecular complex 3, 4, 5 and 6, with no significant difference. Conclusion Molecular complex 1 is mainly related to p53 signaling pathway and cytokine regulation, and is related to bladder cancer. [WT5HZ] [WT5BZ] [WT5BZ] [WT5BZ] [WT5 "HZ]. Molecular complex 2 is involved in many pathways, indicating that synovial sarcoma is a complex process controlled by a network of multiple cellular pathways and multiple genes.
【作者单位】：潍坊医学院临床医学院;潍坊市中医院肿瘤中心;山东中医药大学研究生院;
【基金】：国家自然科学基金面上项目(81673799)
【分类号】：R738.5

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