乳腺癌干细胞BLM、RecQ4蛋白表达变化及意义

发布时间：2018-04-02 23:35

本文选题：乳腺癌　切入点：无血清培养　出处：《山东医药》2017年04期

【摘要】：目的探讨乳腺癌干细胞BLM、RecQ4蛋白表达变化及其临床意义。方法采用无血清培养法从乳腺癌MDA-MB-435细胞中分离乳腺癌干细胞(以下称干细胞),并鉴定。观察MDA-MB-435细胞裸鼠及干细胞成瘤能力,RT-PCR法检测二者ATP结合盒膜转运蛋白G2(ABCG2)mRNA相对表达量,Western blotting法和免疫组化法检测二者BLM、RecQ4蛋白表达。结果干细胞膜表面CD44+CD24-/low比例明显高于、CD44+CD24+比例明显低于MDA-MA-435细胞(P均0.05);两种细胞膜表面CD44-CD24+、CD44-CD24-比例比较差异均无统计学意义(P均0.05)。裸鼠成瘤实验结果显示,与接种相同密度MDA-MB-435细胞比较,接种干细胞的裸鼠成瘤时间早、肿瘤体积大;干细胞ABCG2 mRNA相对表达量为0.753±0.025,MDA-MB-435细胞为0.563±0.112;两者比较P0.05。干细胞BLM蛋白表达低、RecQ4蛋白表达高于MDA-MB-435细胞(P均0.05)。结论乳腺癌干细胞成瘤能力强;细胞BLM蛋白表达降低、RecQ4蛋白表达升高可能为其机制。
[Abstract]:Objective to investigate the expression and clinical significance of BLMtR RecQ 4 protein in breast cancer stem cells.Methods Breast cancer stem cells were isolated and identified from breast cancer MDA-MB-435 cells by serum-free culture.The relative expression of ATP binding cassette transporter (G2(ABCG2)mRNA) in nude mice and stem cells was detected by RT-PCR, and the expression of BLMN RecQ4 protein was detected by immunohistochemistry and Western blotting.Results the ratio of CD44 CD24-/low on the surface of dry cell membrane was significantly higher than that on MDA-MA-435 cell surface (P < 0.05), and there was no significant difference in the ratio of CD44-CD24 CD44-CD24- between the two kinds of cell surface.Compared with MDA-MB-435 cells of the same density, the tumorigenic time of nude mice inoculated with stem cells was earlier and the tumor volume was larger, and the relative expression of ABCG2 mRNA in stem cells was 0.753 卤0.025 MDA-MB-435 cells (0.563 卤0.112), compared with that of P0.05 cells.The expression of BLM protein in stem cells was lower than that in MDA-MB-435 cells (P < 0.05).Conclusion the tumor forming ability of breast cancer stem cells is strong, and the decrease of BLM protein expression and the increase of RecQ4 protein expression may be the mechanism.
【作者单位】：贵州医科大学;
【基金】：国家自然科学基金资助项目(81360349) 贵州省优秀科技教育人才省长专项基金资助项目[黔科合字(2006)57号]
【分类号】：R737.9

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