MiR-214对人乳腺癌MCF-7细胞生物学行为的影响

发布时间：2018-04-04 01:17

本文选题：乳腺癌　切入点：微小RNA　出处：《郑州大学学报(医学版)》2017年01期

【摘要】：目的:探讨微小RNA-214(miR-214)对人乳腺癌MCF-7细胞增殖、凋亡、迁移及周期分布等生物学行为的影响。方法:采用脂质体转染法将miR-214模拟物转入MCF-7细胞(实验组),以转入miR-214阴性对照的MCF-7细胞(阴性对照组)作对照。采用实时荧光定量PCR法检测miR-214的表达情况;CCK-8法检测细胞增殖能力的改变;流式细胞仪检测细胞凋亡及细胞周期;划痕愈合实验检测细胞的迁移。结果:实验组MCF-7细胞miR-214的表达较阴性对照组明显上调;实验组细胞的增殖能力明显受到抑制;实验组凋亡细胞所占比例明显高于阴性对照组(P=0.008),G1期细胞数增加,S期细胞数减少(P0.05);划痕愈合实验表明,实验组细胞迁移能力明显受到抑制(P0.001)。结论:MiR-214可能通过抑制乳腺癌MCF-7细胞增殖及迁移、促进细胞凋亡及影响细胞周期的分布而发挥抑癌作用。
[Abstract]:Aim: to investigate the effects of microRNA-214miR-214) on the proliferation, apoptosis, migration and cycle distribution of human breast cancer MCF-7 cells.Methods: miR-214 mimics were transfected into MCF-7 cells by liposome transfection (experimental group) and miR-214 negative control MCF-7 cells (negative control group) as control.Real-time fluorescence quantitative PCR assay was used to detect the expression of miR-214. CCK-8 method was used to detect cell proliferation; flow cytometry was used to detect cell apoptosis and cell cycle; scratch healing test was used to detect cell migration.Results: the expression of miR-214 in MCF-7 cells in the experimental group was significantly up-regulated than that in the negative control group, and the proliferation ability of the cells in the experimental group was significantly inhibited.The percentage of apoptotic cells in the experimental group was significantly higher than that in the negative control group.Conclusion the cell proliferation and migration of breast cancer MCF-7 cells may be inhibited by the inhibition of cell apoptosis and cell cycle distribution by inhibiting the proliferation and migration of breast cancer MCF-7 cells.
【作者单位】：河北医科大学第一医院普外科;
【基金】：河北省科技支撑计划项目14277755D
【分类号】：R737.9

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