HMGB1过表达和沉默影响子宫内膜癌HEC-1A细胞增殖和侵袭及其机制

发布时间：2018-04-05 13:28

本文选题：高迁移率族蛋白　切入点：子宫内膜癌　出处：《中南大学学报(医学版)》2017年07期

【摘要】：目的:探讨HMGB1基因过表达和沉默对子宫内膜癌增殖与侵袭能力的影响及其机制。方法:构建含有HMGB1重组质粒和HMGB1 sh RNA的慢病毒载体并转染人子宫内膜癌HEC-1A,建立过表达和沉默HMGB1基因的HEC-1A细胞株。采用细胞增殖/毒性活性检测试剂盒(cell counting kit-8)、Transwell小室、细胞划痕实验分析HMGB1过表达和沉默对子宫内膜癌HEC-1A细胞生长、增殖、侵袭及转移的影响。采用Western印迹和反转录PCR(reverse transcriptionPCR,RT-PCR)检测过表达和沉默HMGB1后HEC-1A细胞中NF-κB,VEGF及基质金属蛋白酶2(matrix metalloproteinase2,MMP2)的表达情况。结果:过表达HMGB1能够促进子宫内膜癌HEC-1A细胞增殖、侵袭与转移,且HEC-1A细胞中NF-κB,VEGF及MMP2表达上调;干扰HMGB1表达抑制HEC-1A细胞增殖、侵袭与转移,HEC-1A细胞中NF-κB,VEGF及MMP2表达下调。结论:HMGB1与子宫内膜癌增殖、侵袭与转移密切相关,且可能通过NF-κB,VEGF及MMP2影响子宫内膜癌的侵袭与转移;HMGB1可能成为治疗子宫内膜癌的一个重要靶点。
[Abstract]:Aim: to investigate the effect and mechanism of overexpression and silencing of HMGB1 gene on proliferation and invasion of endometrial carcinoma.Methods: the lentivirus vector containing HMGB1 recombinant plasmid and HMGB1 sh RNA was constructed and transfected into human endometrial carcinoma HEC-1A. The HEC-1A cell line with overexpression and silencing of HMGB1 gene was established.Cell proliferation / toxicity assay kit was used to detect the cell counting kit-8 and transwell chamber. The effects of HMGB1 overexpression and silencing on the growth, proliferation, invasion and metastasis of endometrial carcinoma HEC-1A cells were analyzed by scratch assay.Western blot and reverse transcription PCR(reverse transcriptionPCRRT (RT-PCR) were used to detect the expression of NF- 魏 B Western and matrix metalloproteinase 2(matrix metalloproteinase 2 (MMP2) in HEC-1A cells after HMGB1 was silenced.Results: overexpression of HMGB1 could promote the proliferation, invasion and metastasis of endometrial carcinoma HEC-1A cells, and up-regulate the expression of NF- 魏 B HMGB1 and MMP2 in HEC-1A cells, and interfere with the expression of HMGB1 to inhibit the proliferation of HEC-1A cells and down-regulate the expression of NF- 魏 B _ (1) VEGF and MMP2 in HEC-1A cells.ConclusionHMGB1 is closely related to the proliferation, invasion and metastasis of endometrial carcinoma, and it may be an important target for the treatment of endometrial carcinoma by NF- 魏 B, VEGF and MMP2, which may influence the invasion and metastasis of endometrial carcinoma.
【作者单位】：中南大学湘雅医院妇产科;郴州市第一人民医院南院妇瘤科;湖南省妇幼保健院妇产科;
【基金】：湖南省科学技术厅科技计划一般项目(2014K3125)~~
【分类号】：R737.33

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