中文期刊肿瘤骨转移动物实验研究的报告质量及病理学特征的现状分析

发布时间：2018-04-07 17:10

本文选题：肿瘤骨转移　切入点：动物研究　出处：《兰州大学》2017年硕士论文

【摘要】：目的:1.客观评价2010-2016年中文期刊发表的肿瘤骨转移动物实验研究的报告质量及病理学特征,分析动物实验报告存在的问题,提出解决思路,促进动物实验规范报告;2.针对性地进行有利于科学研究和临床指导的动物实验,为临床研究提供科学合理的,可信度高的动物实验病理评价体系的文献依据。方法:系统、全面地检索中国生物医学文献数据库(Chinese Biomedicine Literature Database,CBM)、相关期刊论文(Chinese Journal Full-text Database,CJFD)、中国科技期刊全文数据库(Chinese Scientific Journal Full-text Database,CSJD)及万方数据库(Wanfang Database),检索时限均从2010年1月至2016年12月,筛选、纳入关于肿瘤骨转移动物实验的研究文献。基于ARRIVE指南、预先设计动物实验资料提取表,对纳入研究的文献进行资料提取,最后用SPSS软件对评估所得的数据进行统计学分析,进一步对纳入研究的病理学检测评价做出总结分析。结果:最终纳入符合标准的234篇肿瘤骨转移动物实验研究文献。234篇文献在背景部分均未对所用动物种类和模型的选择依据进行诠释;仅有3篇(1.28%)在动物实验中提及实验动物的待遇,样本量中没有一篇解释动物实验所需样本量的算法及计算公式;结果部分中仅43篇(18.37%)提供了完整的基线数据,讨论部分中只有30篇(12.80%)评价了研究的局限性。2010-2016年所纳入的动物实验设计样本量普遍较小,肿瘤骨转移动物实验建模类型多采用局部骨内注射制作骨转移模型方式,绝大多数动物实验采用标准合格的方法造模,检测肿瘤骨转移动物实验的造模成功与否和抗肿瘤药物疗效的方法不太完善。病理学检测评价中少部分对染色和检测手段过程描述不太清晰,检测方法不太全面。结论:1.中文期刊中肿瘤骨转移的动物实验未能完整报告实验研究的相关重要信息;2.中文期刊中肿瘤骨转移动物实验造模方法规范,检测肿瘤骨转移动物实验的造模成功与否和抗肿瘤药物疗效的检测方法有待更加完善;3.基于中文期刊中肿瘤骨转移动物实验病理评价结果,为建立科学合理的病理评价体系提供了文献依据。
[Abstract]:Purpose 1.Objective to evaluate the quality and pathological characteristics of the animal experimental study of tumor bone metastasis published in Chinese Journal 2010-2016, analyze the problems existing in the animal experimental report, and put forward solutions to promote the standard report of animal experiment.Animal experiments, which are beneficial to scientific research and clinical guidance, are carried out in order to provide scientific, reasonable and reliable literature basis for the pathological evaluation system of animal experiments.Methods: system,Into the research literature on tumor bone metastasis animal experiment.Based on the ARRIVE guide, the extraction table of animal experimental data was designed in advance, and the data of the literature included in the study were extracted. Finally, the data obtained from the evaluation were statistically analyzed by SPSS software.Further, the pathological examination and evaluation included in the study were summarized and analyzed.Results: finally, 234 articles of animal experimental study of tumor bone metastasis were included in the standard. In the background part, none of them explained the selection basis of the animal species and models used.Only three articles 1.28 mentioned the treatment of experimental animals in animal experiments, and none of the samples explained the algorithms and formulas for calculating the amount of samples required for animal experiments; the result was only 43 parts 18.37) providing complete baseline data.In the discussion section, only 30 articles (12.80) evaluated the limitation of the study. The sample size of animal experimental design for 2010-2016 was generally small, and most animal models of tumor bone metastasis were made by local intraosseous injection.Most animal experiments were made by standard and qualified methods, and the methods of detecting the success of animal models of tumor bone metastasis and the efficacy of antitumor drugs were not perfect.In the evaluation of pathological examination, the description of the process of staining and detection is not clear, and the detection method is not very comprehensive.Conclusion 1.Animal experiments of tumor bone metastasis in Chinese journals fail to report relevant important information on experimental studies. 2.The standard of animal model of tumor bone metastasis in Chinese Journal, the method to detect the success of tumor bone metastasis animal experiment and the detection method of anti-tumor drug efficacy need to be improved.Based on the results of experimental pathological evaluation of bone metastases in Chinese journals, a scientific and reasonable pathological evaluation system was established.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R730.5

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