miR-370和EGFR在肺癌中的表达水平及其临床意义研究

发布时间：2018-04-09 06:38

本文选题：miR-370　切入点：EGFR　出处：《昆明医科大学》2017年硕士论文

【摘要】：[目的]1、明确miR-370、EGFR在肺癌组织中的表达水平。2、明确在肺癌组织中miR-370和EGFR之间是否有相关性。3、通过肺癌患者癌组织中miR-370的表达水平与其临床病理特征的相关性分析,探索miR-370的表达水平是否与临床病理特征相关及其临床意义,为开发肺癌新的治疗靶点提供理论依据。[方法]1、收集38例肺癌患者肺组织,每例患者分别取正常组织(在离肿瘤边缘最远端取材)和癌组织(肿瘤中心取材,避免取材坏死部分)。分别提取两组组织中的总RNA,逆转录为cDNA,采用实时荧光定量PCR检测两组组织中miR-370和EGFR表达水平的高低,用2-△△CT计算miR-370和EGFR的相对表达量。2、分析miR-370和EGFR的表达水平是否有相关性。3、分析miR-370在肺癌患者癌组织中的表达水平与其临床特征、肿瘤标志物及病理参数之间的关系。4、统计学分析:数据处理用SPSS 21.0统计软件包。差异性表达用Wilcoxon符号秩检验进行统计,相关性分析用Spearman进行检验。[结果]1、miR-370在肺癌患者正常组织中的表达水平高于其在癌组织中的表达水平,差异有统计学意义(p=0.0160.05)。2、EGFR在肺癌患者癌组织中的表达水平显著高于其在正常组织中的表达水平,差异有统计学意义(p=0.0080.05)。3、miR-370和EGFR在肺癌组织中的表达水平呈负相关性(r=-0.333,p=0.0030.05)。4、miR-370在肺癌患者癌组织中的表达水平越高非腺癌的倾向性越高(r=0.229,p=0.0460.05); miR-370的表达水平与NSE呈正相关(r=0.326, p=0.0240.05);与 SCC 呈正相关(r=0.285,p=0.0270.05)。5、miR-370在肺癌患者癌组织中的表达水平与患者的年龄、性别、地域、肿瘤大小、分期、淋巴结转移和远处转移情况无相关性(p0.05);与CEA、CA125、CA153、CA199、CA242、CYFRA21-1等肿瘤标志物无相关性(p0.05)。[结论]1、miR-370在肺癌患者正常组织中的表达水平高于其在癌组织中的表达水平。2、EGFR在肺癌患者癌组织中的表达水平显著高于其在正常组织中的表达水平。3、miR-370和EGFR在肺癌组织中的表达水平呈负相关,提示EGFR可能是miR-370调控肺癌的一个靶基因。4、miR-370在肺癌患者癌组织中的表达水平越高非腺癌的倾向性越高,miR-370的表达水平与NSE呈正相关,与SCC呈正相关,提示miR-370可能与肺癌的组织学分类有关。
[Abstract]:[objective] 1. To determine the expression level of miR-370EGFR in lung cancer tissues, and to determine whether there is a correlation between miR-370 and EGFR in lung cancer tissues, and to analyze the correlation between the expression level of miR-370 and clinicopathological features of lung cancer patients.To explore whether the expression of miR-370 is related to clinicopathological features and its clinical significance, to provide theoretical basis for the development of new therapeutic targets for lung cancer.[methods] 1. The lung tissues of 38 patients with lung cancer were collected. The normal tissues (at the farthest edge of the tumor) and the cancer tissues (the tumor center) were taken from each patient to avoid necrotic parts.Total RNAs were extracted from two groups of tissues and reverse transcripted to cDNA.Real-time fluorescence quantitative PCR was used to detect the expression of miR-370 and EGFR in the two groups.The relative expression of miR-370 and EGFR was calculated by 2-CT. The correlation between miR-370 and EGFR was analyzed, and the expression of miR-370 in lung cancer was analyzed.The relationship between tumor markers and pathological parameters. 4. Statistical analysis: SPSS 21. 0 statistical software package was used for data processing.The difference expression was analyzed by Wilcoxon sign rank test and correlation analysis by Spearman.[results] 1the expression level of miR-370 in normal tissues of lung cancer was higher than that in normal tissues of lung cancer, and the difference was statistically significant (P < 0.05). The expression level of EGFR in lung cancer was significantly higher than that in normal tissues.There was a positive correlation between the expression of SCC and the age of patients with lung cancer.There was no correlation between sex, region, tumor size, stage, lymph node metastasis and distant metastasis (p0.05), but no correlation with tumor markers such as CA242CYFRA21-1.[conclusion] 1 the expression level of miR-370 in normal tissues of lung cancer patients is higher than that in normal tissues of lung cancer patients. 2EGFR expression level in cancer tissues of lung cancer patients is significantly higher than that in normal tissues. 3 miR-370 and EGFR expression level in lung cancer group.The level of expression in weaving was negatively correlated.It is suggested that EGFR may be a target gene of miR-370 regulating lung cancer. The higher the expression level of MR-370 in lung cancer is, the higher the tendency of non-adenocarcinoma is. The higher the expression level of miR-370 is, the positive correlation is between the expression of MR-370 and NSE, and the expression level of MR-370 is positively correlated with SCC.The results suggest that miR-370 may be related to the histological classification of lung cancer.
【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R734.2

【参考文献】