4种人肾细胞癌原位移植裸鼠模型建立方法的比较

发布时间：2018-04-15 11:28

本文选题：肾细胞癌 + 裸鼠　；参考：《中国癌症杂志》2017年03期

【摘要】：背景与目的:肾细胞癌是最常见的肾脏恶性肿瘤,起病隐匿,恶性程度高。研究旨在建立成功率高、稳定的人肾细胞癌原位动物造模方法。方法:采用人肾细胞癌细胞(786-0、ACHN)进行细胞悬液原位注射法、皮下成瘤后引瘤原代细胞悬液原位注射法、皮下成瘤后引瘤组织块原位移植肾包膜下法及皮下成瘤后引瘤组织块原位移植肾周筋膜内法建立原位移植裸鼠模型,采用PET/CT、H-E染色、免疫组织化学染色及血清生化检测等手段观察成瘤率及肿瘤生长情况,评估4种方法的建模效果。结果:人肾细胞癌皮下移植瘤裸鼠模型,ACHN组成瘤率(90%)高于786-0组(30%);人肾细胞癌原位移植裸鼠模型,786-0原位注射组、ACHN原位注射组、ACHN皮下移植瘤引瘤后原代细胞原位注射组、ACHN组织块肾包膜下包埋组和ACHN组织块肾周筋膜内包埋组的成瘤率分别为33%、80%、90%、100%和20%,其中以ACHN皮下成瘤后引瘤组织块原位移植肾包膜下法成瘤率最高,影像学及病理组织学检查结果显示符合低分化肾细胞癌。结论:成功建立4种人肾细胞癌原位移植裸鼠模型,其中以ACHN皮下成瘤后引瘤组织块原位移植肾包膜下法成瘤效果最佳,为肾细胞癌发病机制及靶向治疗的进一步研究提供理想的动物模型。
[Abstract]:Background & objective: renal cell carcinoma (RCC) is the most common renal malignancy with hidden onset and high malignancy.The aim of this study was to establish an in situ animal model of human renal cell carcinoma (RCC) with high success rate.Methods: in situ injection of human renal cell carcinoma cell line 786-0 ACHN was performed, and the primary cell suspension after subcutaneous tumorigenesis was injected in situ.Nude mice models of orthotopic transplantation were established by orthotopic transplantation of subcutaneous tumor tissue mass under renal capsule and subcutaneous tumor tissue mass orthotopic transplantation of perirenal fascia, and PET / CTH-E staining was used.The tumor formation rate and tumor growth were observed by immunohistochemical staining and serum biochemical detection, and the modeling effects of the four methods were evaluated.Results: the tumor rate of ACHN in nude mice with subcutaneous transplanted tumor of human renal cell carcinoma was 90% higher than that in group 786-0, and that in nude mice model of orthotopic transplantation of human renal cell carcinoma was 786-0.The tumorigenic rate of the subcapsular embedding group and the perirenal fascial embedding group of ACHN tissue mass were 338090% and 20%, respectively. Among them, the rate of tumor formation was the highest in the subcapsular transplantation with ACHN subcutaneous tumorigenesis and orthotopic transplantation of the tumor tissue mass, and the rate of tumor formation was the highest in the ACHN tissue mass perirenal intrafascial embedding group, and the tumor forming rate was the highest in the subcapsular implantation group after ACHN subcutaneous tumor formation.The results of imaging and histopathology showed that it was consistent with poorly differentiated renal cell carcinoma.Conclusion: four orthotopic transplantation models of human renal cell carcinoma (RCC) were successfully established in nude mice, in which ACHN subcutaneous tumorigenesis was the most effective method for renal transplantation.To provide an ideal animal model for the further study of the pathogenesis and targeted therapy of renal cell carcinoma.
【作者单位】：上海交通大学医学院附属仁济医院肾脏科;上海交通大学医学院附属仁济医院中心实验室;
【基金】：上海市科学技术委员会科研计划项目(15140902700)
【分类号】：R-332;R737.11

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