替莫唑胺对人脑胶质瘤细胞U251增殖、凋亡的影响及其机制探讨

发布时间：2018-04-15 18:39

本文选题：替莫唑胺 + 人脑胶质瘤细胞U　；参考：《山东医药》2017年47期

【摘要】：目的观察替莫唑胺对人脑胶质瘤细胞U251增殖、凋亡的影响,并探讨其可能机制。方法取对数生长期U251细胞,分别加入0.02、0.06、0.1 g/L替莫唑胺,空白对照组不加替莫唑胺,分别培养24、48、72 h,MTT实验观察细胞增殖情况;取对数生长期U251细胞,加入0.06 g/L替莫唑胺(0.06 g/L替莫唑胺组),细胞培养48 h后,用流式细胞技术测算细胞凋亡率,RT-PCR法、Western blotting法检测存活素(Survivin)mRNA、蛋白。结果随着替莫唑胺浓度增加及作用时间延长,U251细胞增殖抑制率越高。0.06 g/L替莫唑胺组细胞凋亡率为22.6%±2.3%,对照组为3.3%±1.1%,两组比较,P0.05;0.06 g/L替莫唑胺组Survivin mRNA相对表达量为0.65±0.11,对照组为0.96±0.15,两组比较,P0.05;0.06 g/L替莫唑胺组Survivin蛋白相对表达量为0.25±0.03,对照组为0.49±0.04,两组比较,P0.05。结论替莫唑胺能有效抑制U251细胞增殖,诱导细胞凋亡,其机制可能与抑制Survivin表达有关。
[Abstract]:Aim to observe the effect of temozolidomide on the proliferation and apoptosis of human glioma cell line U251 and to explore its possible mechanism.Methods U251 cells in logarithmic growth phase were added 0.02 0. 06 ~ 0. 1 g / L temozolidomide, respectively. The control group was treated without temozolidomide, and the proliferation of U251 cells in logarithmic growth phase was observed by MTT assay for 72 h, and the proliferation of U251 cells in logarithmic growth phase was observed by MTT assay.0.06 g / L temozolidomide 0.06 g / L temozolidomide group was added. After 48 hours of cell culture, cell apoptosis rate was measured by flow cytometry. Survivin survivin mRNAs and proteins were detected by RT-PCR and Western blotting.Results the proliferation inhibition rate of U251 cells increased with the increase of temozolidomide concentration and the time of treatment. The apoptosis rate of U251 cells was 22.6% 卤2.3% and 3.3% 卤1.1 in the control group and the control group, respectively. The relative expression of Survivin mRNA was 0.65 卤0.11 in the P0.050.06 g / L / L temozolidomide group and the control group.The relative expression of Survivin protein was 0.25 卤0.03 in the two groups and 0.49 卤0.04 in the control group.Conclusion temozolomide can effectively inhibit the proliferation and induce apoptosis of U251 cells. The mechanism may be related to the inhibition of Survivin expression.
【作者单位】：新乡医学院第三附属医院;新乡医学院药学院;
【分类号】：R739.41

【相似文献】