PES1和EGFR在胃癌组织中的表达及其临床意义

发布时间：2018-04-16 07:22

  本文选题：PES1 + EGFR　； 参考：《青岛大学》2017年硕士论文

【摘要】：目的检测PES1及EGFR在胃癌组织中的表达,探讨PES1和EFGR与胃癌的各种临床病理特征的关系及两者在胃癌组织中表达的相关性。方法收集60例胃癌患者的外科手术切除标本(60例患者均无肿瘤家族史,无遗传病史,术前均未曾接受过化疗及放疗等抗癌治疗措施),取材、包埋、切片后,进行常规HE染色,整理患者的年龄、性别、肿瘤分化程度、肿瘤浸润深度、临床病理分期、淋巴结转移情况等临床病理资料。根据HE染色切片的诊断结果,选定典型的蜡块,采用手工S-P两步法进行免疫组织化学染色,检测PES1和EGFR在胃癌及癌旁组织中的表达情况。将表达结果按照规定的标准进行判读,整理数据,将数据输入SPSS17.0统计软件进行统计学的分析处理。结果①PES1在60例胃癌组织中阳性表达率(31.67%)明显高于癌旁组织(3.33%),差异具有统计学意义(x~2=16.681,P0.05);EGFR在胃癌组织中阳性表达率(75%)也明显高于癌旁组织(10%),差异具有统计学意义(x~2=51.867,P0.05)。②PES1和EGFR在胃癌组织中表达无相关性(r=0.062,P=0.873)。③PES1在胃癌组织中的高表达与肿瘤的浸润深度(x~2=7.288,P0.05)和临床病理分期(x~2=8.182,P0.05)密切相关,在侵达浆膜层者阳性率(41.86%)明显高于未侵达浆膜层者的阳性率(5.88%),在胃癌临床病理分期为Ⅲ-Ⅳ期患者的阳性率(44.74%)明显高于Ⅰ-Ⅱ期患者的阳性率(9.09%);而与患者的年龄(x~2=0.184)、性别(x~2=0.026)、肿瘤分化程度(x~2=0.255)和淋巴结转移情况(x~2=0.490)无相关性(P0.05)。(4)EGFR在胃癌组织中的高表达与肿瘤的浸润深度(x~2=17.100,P0.05)、临床病理分期(x~2=27.656,P0.05)和淋巴结转移(x~2=31.801,P0.05)密切相关,在侵达浆膜层患者阳性率(90.70%)明显高于未侵达浆膜层者的阳性率(35.29%),在临床病理分期Ⅲ-Ⅳ期的胃癌患者中的阳性率(97.37%)明显高于Ⅰ-Ⅱ期患者的阳性率(36.36%),在有淋巴结转移者的阳性表达率(93.48%)高于无淋巴结转移者的阳性率(14.29%);而与患者的性别(x~2=0.030)、年龄(x~2=2.262)、肿瘤分化程度(x~2=0.044)无相关性(P0.05)。结论PES1与EGFR在60例胃癌组织中的阳性表达率均明显高于癌旁组织;两者在胃癌组织中的表达无相关性;PES1在胃癌组织中的高表达与肿瘤浸润深度、临床病理分期密切相关,而与患者的年龄、性别、肿瘤分化程度、有无淋巴结转移等情况无关;EGFR在胃癌组织中的高表达与肿瘤的浸润深度、临床病理分期、淋巴结转移情况有关,与患者的年龄、性别、肿瘤分化程度等临床病理参数无关。综上所述,PES1和EGFR在胃癌的发生发展中起重要作用,是胃癌治疗的重要靶点,同时也是胃癌预后评估的重要参考因素。
[Abstract]:Objective to detect the expression of PES1 and EGFR in gastric cancer and to explore the relationship between PES1 and EFGR and the clinicopathological features of gastric cancer.Methods A total of 60 patients with gastric cancer underwent surgical excision without a family history of cancer, no history of hereditary diseases, no chemotherapy and radiotherapy before operation, and no samples taken, embedded, sliced, and then stained with HE.Age, sex, tumor differentiation, depth of tumor invasion, clinicopathologic stage, lymph node metastasis and other clinicopathological data were analyzed.According to the diagnostic results of HE staining sections, the typical wax blocks were selected and the expression of PES1 and EGFR in gastric cancer and adjacent tissues was detected by using the two-step immunohistochemical method of S-P.The expression result is interpreted according to the prescribed standard, the data is sorted out, and the data is input into the SPSS17.0 statistical software for statistical analysis and processing.Results the positive expression rate of 1PES1 in 60 cases of gastric cancer was significantly higher than that in adjacent tissues (3.33%), and the difference was statistically significant. The positive expression rate of 1PES1 in gastric cancer tissues was significantly higher than that in adjacent tissues of gastric cancer (P 0.052.The difference was statistically significant.There was no correlation between the expression of PES1 and EGFR in gastric cancer tissues. The high expression of PES1 and EGFR in gastric cancer tissues was closely related to the depth of invasion of the tumor and the clinicopathologic stage (P0.05).The positive rate in patients with invasion of serous layer (41.86) was significantly higher than that in patients without serous layer (5.88%), and the positive rate in patients with stage 鈪，

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