YAP,β-catenin在乳腺浸润性导管癌中表达及其临床意义

发布时间：2018-04-16 07:40

本文选题：乳腺浸润性导管癌 + YAP　；参考：《兰州大学》2015年硕士论文

【摘要】：目的：分别检测乳腺浸润性导管癌(非特殊型)、乳腺腺瘤以及正常乳腺组织中YAP和β-catenin的表达情况,以此分析YAP、β-catenin二者与乳腺浸润性导管癌临床病理因素之间的关系、表达的相关性、与乳腺浸润性导管癌预后的关系,进一步探讨YAP、β-catenin两个蛋白与乳腺浸润性导管癌的发生、发展及预后的关系。方法：采用免疫组化SP法检测YAP和β-catenin在32例正常乳腺组织、40例乳腺腺瘤组织及97例乳腺浸润性导管癌组织中的表达情况。结果：(1)YAP在正常乳腺组织中的表达明显高于乳腺腺瘤组织及乳腺浸润性导管癌组织,其在正常乳腺组织、乳腺腺瘤组织及乳腺浸润性导管癌组织中的阳性表达率依次减低,差异有统计学意义(P0.05)。在乳腺浸润性导管癌组织中,β-catenin的表达情况明显高于在正常乳腺组织的表达,也高于在乳腺腺瘤组织的表达。β-catenin在正常乳腺组织、乳腺腺瘤组织及乳腺浸润性导管癌组织中的阳性表达率依次上调,差异有统计学意义(P0.05)。(2)YAP表达与乳腺浸润性导管癌患者年龄、组织学分级、肿瘤大小、淋巴结转移情况、是否绝经等均无相关性(P0.05);与是否为三阴性乳腺癌相关(P0.05)。β-catenin表达与患者年龄、肿瘤大小、是否绝经等均无相关性(P0.05)；与组织学分级、淋巴结转移情况、是否为三阴性乳腺癌相关(P0.05)。(3)YAP的阳性表达与乳腺浸润性导管癌患者的总生存率不相关(P0.05)；与无复发生存率相关(P0.05)。β-catenin阳性表达与乳腺浸润性导管癌患者的总生存率、无复发生存率均不相关(P0.05)。YAP(-)/β-catenin(+)共表达与乳腺浸润性导管癌患者的总生存率、无复发生存率均相关(P0.05)。(4)在乳腺浸润性导管癌、乳腺腺瘤组织中YAP和β-catenin的表达均呈负相关(rs为负值,P0.01)；YAP和β-catenin二者在正常乳腺中的表达无相关性(P0.05)。结论：(1)β-catenin在乳腺浸润性导管癌组织中呈高表达,并与淋巴结转移情况相关,提示βcatenin对乳腺浸润性导管癌的发生和转移有促进作用。(2)β-catenin在乳腺浸润性导管癌组织中的表达情况与组织分级有关,提示β-catenin可作为乳腺浸润性导管癌组织分级判断的参考指标。(3)在乳腺浸润性导管癌组织中YAP和β-catenin的表达呈负相关,且YAP(-)/β-catenin(+)共表达与乳腺浸润性导管癌患者的总生存率、无复发生存率均相关,提示二者联合检测有助于乳腺浸润性导管癌的临床诊断、疗效评估及预后判断。
[Abstract]:Objective: to detect the expression of YAP and 尾 -catenin in invasive ductal carcinoma of breast (non-special type, adenoma and normal mammary gland), and to analyze the relationship between YAPand 尾 -catenin and clinicopathological factors of invasive ductal carcinoma of breast.The relationship between YAPand 尾 -catenin protein and the occurrence, development and prognosis of invasive ductal carcinoma of breast were discussed.Methods: immunohistochemical SP method was used to detect the expression of YAP and 尾 -catenin in 32 cases of normal breast tissues, 40 cases of breast adenoma and 97 cases of breast invasive ductal carcinoma.Results the expression of yap in normal breast tissue was significantly higher than that in adenoma tissue and invasive ductal carcinoma tissue, and the positive expression rate in normal breast tissue, breast adenoma tissue and invasive ductal carcinoma tissue was decreased in turn.The difference was statistically significant (P 0.05).The expression of 尾 -catenin in breast invasive ductal carcinoma was significantly higher than that in normal breast and adenoma.The positive expression rate in breast adenoma and breast invasive ductal carcinoma was up-regulated in turn. The difference was statistically significant between the expression of YAP and age, histological grade, tumor size, lymph node metastasis in patients with invasive ductal carcinoma of breast.The expression of 尾 -catenin was not correlated with age, tumor size, menopause, histological grade and lymph node metastasis.There was no correlation between the positive expression of P0.05A and the overall survival rate of breast invasive ductal carcinoma patients, and the correlation between the positive expression of 尾 -catenin and the overall survival rate of breast infiltrating ductal carcinoma patients was not correlated with the recurrence free survival rate (P 0.05). The positive expression of 尾 -catenin was associated with the overall survival rate of invasive ductal carcinoma of the breast.The recurrence free survival rate was not correlated with the overall survival rate of breast invasive ductal carcinoma patients, and the recurrence free survival rate was correlated with the P0.05P / 尾 -catenin (尾 -catenin (尾 -catenin) co-expression) in invasive ductal carcinoma of the breast.The expression of YAP and 尾 -catenin in breast adenoma was negatively correlated with the expression of 尾 -catenin. There was no correlation between the expression of YAP and 尾 -catenin in normal mammary gland.Conclusion 尾 -catenin is highly expressed in breast invasive ductal carcinoma, and is related to lymph node metastasis.It is suggested that 尾 catenin can promote the occurrence and metastasis of invasive ductal carcinoma of breast. The expression of 尾 -catenin in breast invasive ductal carcinoma is related to the histological grade.The results suggest that 尾 -catenin can be used as a reference index for grading of invasive ductal carcinoma of breast. The expression of YAP and 尾 -catenin in invasive ductal carcinoma of breast is negatively correlated with the expression of 尾 -catenin (YAP(-)/ 尾 -catenin), and the overall survival rate of patients with invasive ductal carcinoma of breast is associated with the co-expression of YAP(-)/ 尾 -catenin (YAP(-)/ 尾 -catenin (尾 -catenin)).The recurrence free survival rate was correlated, suggesting that the combined detection was helpful for the clinical diagnosis, evaluation of curative effect and prognosis of invasive ductal carcinoma of breast.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R737.9

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