乳腺癌组织Notch1和JAG1蛋白表达与分子分型相关性研究

发布时间：2018-04-18 10:39

本文选题：乳腺肿瘤 + 分子分型　；参考：《中华肿瘤防治杂志》2017年05期

【摘要】：目的 Notch信号通路在乳腺癌中存在异常表达,但在不同分子分型乳腺癌中的表达情况鲜见报道。本研究将探讨不同分子分型乳腺浸润性导管癌组织中Notch1和JAG1蛋白表达及其与临床病理特征之间的关系。方法收集滨州医学院附属医院病理科2012-01-06-2014-12-30存档的乳腺浸润性导管癌病例240例,根据ER、PR、HER2、Ki-67免疫组化结果分为管腔A型、管腔B型、HER2过表达型及三阴性型4组,每组60例,采用免疫组化En-Vision法检测不同分子分型乳腺癌中Notch1、JAG1蛋白的表达情况,对各分型乳腺癌组织的阳性表达率进行统计学分析。结果各分型乳腺癌组织中Notch1的阳性表达差异有统计学意义(P=0.010,P0.05),其中三阴性乳腺癌(86.67%)与管腔A型(56.67%)比较(P0.001),HER2过表达型(83.33%)与管腔A型(56.67%)比较,差异均有统计学意义,P0.001;Notch1的阳性表达与淋巴结转移相关(P=0.017,P0.05),与临床分期(P=0.005,P0.01)、组织学分级(P=0.009,P0.01)显著相关,与ER表达(r_s=-0.206,P=0.001,P0.01)、PR表达(r_s=-0.187,P=0.005,P0.01)显著负相关,而与患者年龄、肿块大小无关。各分型乳腺癌组织中JAG1的阳性表达差异有统计学意义(P=0.035,P0.05),以三阴性乳腺癌阳性表达率最高,但各组间两两比较差异无统计学意义;JAG1阳性表达与ER表达(r_s=-0.142,P=0.015,P0.05)、PR表达(r_s=-0.127,P=0.035,P0.05)呈负相关;Notch1和JAG1之间无明显相关性。结论 Notch1阳性表达与ER、PR表达显著负相关,与乳腺癌的组织学分级、淋巴结转移及临床分期密切相关,并在HER2过表达型,尤其是三阴性乳腺癌组织中存在高表达,有可能成为三阴性乳腺癌新的治疗靶点。
[Abstract]:Objective there is abnormal expression of Notch signaling pathway in breast cancer, but it is rarely reported in different molecular types of breast cancer.The purpose of this study was to investigate the expression of Notch1 and JAG1 in breast invasive ductal carcinoma with different molecular types and their relationship with clinicopathological features.Methods 240 cases of invasive ductal carcinoma of breast were collected from Department of Pathology, affiliated Hospital of Binzhou Medical College, from January to June 2014-12-30. According to the immunohistochemical results of ERB HER2 Ki-67, 240 cases were divided into 4 groups: type A, type B, and type B, with 60 cases in each group.Immunohistochemical En-Vision method was used to detect the expression of Notch1 and JAG1 protein in different molecular types of breast cancer, and the positive expression rate of Notch1 and JAG1 protein was analyzed statistically.Results there were significant differences in the positive expression of Notch1 between the three negative breast cancer tissues (P 0.010 and P 0.05, including three negative breast cancer (86.67)) and the lumen A (56.67) (P 0.001 HER2 overexpression type (83.33) and the lumen A (56.67)).There was significant correlation between the positive expression of P0.001ntch1 and lymph node metastasis (P0. 017 / P0. 05), clinical stage P0. 005 / P0. 01, histological grade P0. 009 / P0. 01), and the positive expression of P0. 001- 0. 206P0. 001p0. 01PR was negatively correlated with the age of the patients and the size of the tumor, but it was not correlated with the age of the patients and the size of the tumor. The positive expression of P0. 001ntch1 was significantly correlated with the expression of P0. 017 and P0. 005P0. 01, but not correlated with the age of the patients and the size of the tumor.The positive expression of JAG1 in breast cancer was significantly different among the three types of breast cancer, and the positive expression rate of JAG1 was the highest in triple-negative breast cancer.But there was no significant difference between the two groups. There was no significant correlation between the positive expression of JAG1 and the expression of ER, rstch1 and JAG1. There was no significant correlation between the positive expression of JAG1 and the expression of rsser-0.127 P0. 035 and JAG1.Conclusion the positive expression of Notch1 is negatively correlated with the expression of ERP PR, and is closely related to the histological grade, lymph node metastasis and clinical stage of breast cancer, and is highly expressed in the overexpression of HER2, especially in triple-negative breast cancer.It is possible to become a new therapeutic target for triple negative breast cancer.
【作者单位】：滨州医学院附属医院病理科;滨州医学院病理学教研室;
【基金】：山东省自然科学基金(ZR2010HM096)
【分类号】：R737.9

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