免疫磁珠对肺腺癌小鼠模型micro-CT的增强作用

发布时间：2018-04-18 11:14

本文选题：肺腺癌 + 生物发光成像　；参考：《中国肿瘤临床》2017年12期

【摘要】：目的:研究偶联单抗NJ001的免疫磁珠对肺腺癌裸鼠模型micro-CT扫描的增强作用。方法:经尾静脉注射SPC-A1-luc细胞建立肺腺癌裸鼠模型,生物发光成像定量监测肿瘤的大小。裸鼠分为:生理盐水组、裸磁珠组和免疫磁珠组,每周分别注射生理盐水、750 nm裸磁珠溶液和偶联NJ001的750 nm免疫磁珠溶液,于注射前和注射后4 h进行micro-CT扫描。利用免疫组织化学验证肿瘤组织中NJ001特异性抗原SP70的表达。结果:免疫磁珠组在第4周即可检测到肿瘤,而生理盐水组和裸磁珠组均到第6周才能检测到。第6周生理盐水组、裸磁珠组和免疫磁珠组micro-CT扫描肿瘤的灰度值分别为注射前的59.05±0.66、60.69±0.55和58.25±0.32,注射后的60.30±1.83、61.05±0.68和67.41±3.82。与注射前相比,免疫磁珠组注射后的灰度值显著增加,差异具有统计学意义(P=0.007 9)。生理盐水组和裸磁珠组注射后的灰度值与注射前相比差异均无统计学意义(均P0.05)。结论:偶联单抗NJ001的免疫磁珠对肺腺癌裸鼠模型micro-CT扫描有增强作用,并且有望用于肺癌的早期诊断。
[Abstract]:Aim: to study the enhancement effect of immunomagnetic beads of coupling monoclonal antibody (NJ001) on micro-CT scanning of lung adenocarcinoma in nude mice.Methods: SPC-A1-luc cells were injected into tail vein to establish nude mice model of lung adenocarcinoma and the tumor size was quantitatively monitored by bioluminescent imaging.Nude mice were divided into three groups: saline group, naked magnetic bead group and immunomagnetic bead group. They were injected with normal saline 750nm naked magnetic beads solution every week and 750nm immunomagnetic beads solution coupled with NJ001 respectively. Micro-CT scanning was performed before injection and 4 hours after injection.The expression of NJ001 specific antigen SP70 in tumor tissues was confirmed by immunohistochemistry.Results: the tumor was detected in the immunomagnetic beads group at the 4th week, but not in the saline group and the bare magnetic beads group until the 6th week.At the 6th week, the grayscale values of micro-CT in saline group, bare magnetic bead group and immunomagnetic bead group were 59.05 卤0.66U 60.69 卤0.55 and 58.25 卤0.32, 60.30 卤1.83 卤0.68 and 67.41 卤3.82, respectively.The gray value of immunomagnetic bead group was significantly higher than that before injection, and the difference was statistically significant.There was no significant difference in gray value between saline group and naked magnetic bead group after injection (P 0.05).Conclusion: the immunomagnetic beads of coupling monoclonal antibody NJ001 can enhance the micro-CT scan of lung adenocarcinoma in nude mice and may be used in the early diagnosis of lung cancer.
【作者单位】：南京医科大学第一附属医院检验学部;
【基金】：国家自然科学基金项目(编号:81672100)资助~~
【分类号】：R-332;R734.2

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