sCD40L对白血病Kasumi-1细胞的影响及机制

发布时间：2018-04-18 11:41

本文选题：sCDL + Kasumi-细胞　；参考：《上海交通大学学报(医学版)》2017年01期

【摘要】：目的·探讨可溶性CD40配体(sCD40L)对体外培养白血病Kasumi-1细胞增殖、凋亡的影响及分子机制。方法·用不同梯度浓度sCD40L处理Kasumi-1细胞,在24、48、72 h测定细胞增殖率,以筛选优化的干预浓度和时间。用优化的sCD40L浓度和时间干预处理Kasumi-1细胞后,采用流式细胞术检测细胞凋亡率和细胞周期分布;采用免疫印迹法(Western blotting)检测凋亡因子Cytc、Bax、Bid和Bcl-2的表达;采用ELISA法检测sCD40L处理后Kasumi-1细胞培养上清液中IL-17含量。结果·sCD40L体外最佳干预实验浓度和时间分别为4μg/m L和48 h。经sCD40L处理后,Kasumi-1细胞体外增殖明显受到抑制,同时凋亡率明显升高;细胞周期分布表现为G1期细胞比例增加、S期细胞比例降低;促凋亡因子Bax、Bid、Cytc表达水平明显升高,抗凋亡因子Bcl-2表达水平显著降低;Kasumi-1细胞培养上清液中IL-17含量明显增高。结论·sCD40L可显著抑制Kasumi-1细胞增殖并促进其凋亡,其作用机制与线粒体通路及上调IL-17表达水平有关。
[Abstract]:Objective to investigate soluble CD40 ligand (sCD40L) on proliferation of leukemia Kasumi-1 cells in vitro, apoptosis and molecular mechanisms. Methods with different concentrations of sCD40L Kasumi-1 in 24,48,72 cells, H cell proliferation rates were measured, and when to interfere with concentration screening. Kasumi-1 cells were treated with sCD40L concentration and intervention time optimization after the detection of apoptosis rate and cell cycle distribution by flow cytometry; immunoblotting (Western blotting) detection of apoptotic factor Cytc, Bax, expression of Bid and Bcl-2; the IL-17 content in the supernatant of Kasumi-1 cells treated with sCD40L was detected by ELISA. Results sCD40L in vitro experiments the best intervention time and concentration respectively. 4 g/m L and 48 h. After sCD40L treatment, the proliferation of Kasumi-1 cells was significantly inhibited, and the apoptosis rate increased; the cell cycle distribution of G1 phase cells Increase the proportion of cells in S phase decreased; apoptosis factor Bax, Bid, Cytc expression levels increased significantly, the expression of anti apoptosis factor Bcl-2 levels decreased significantly; Kasumi-1 cells of IL-17 in the supernatant was obviously increased. Conclusion sCD40L can significantly inhibit the proliferation of Kasumi-1 cells and promote its apoptosis, its mechanism and the mitochondrial pathway and upregulation of IL-17 expression levels.

【作者单位】：遵义医学院附属医院血液科;
【基金】：贵州省科技厅联合基金项目[黔科合LH字(2015)7468]~~
【分类号】：R733.7

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