索拉菲尼通过活化p38MAPK抑制人口腔癌TCA8113细胞增殖

发布时间：2018-04-21 07:12

本文选题：索拉菲尼 + TCA　；参考：《实用口腔医学杂志》2017年01期

【摘要】：目的:观察索拉菲尼对人口腔癌TCA8113细胞增殖的影响并探讨其作用机制。方法:以浓度(2.5、5、10、20μg/ml)索拉菲尼干预人口腔癌TCA8113细胞48 h后,以MTT法及集落形成实验检测细胞增殖,蛋白质免疫印迹检测不同浓度拉菲尼对p38MAPK活化的影响;为检测p38MAPK在索拉菲尼抗TCA8113细胞增殖中的作用,先以10μmol/L SB203580(p38MAPK特异性抑制剂)预处理TCA81 13细胞30 min,随后加入不同浓度索拉菲尼继续培养48 h,以MTT法检测细胞增殖情况。结果:索拉菲尼能显著抑制TCA8113细胞的增殖,其抗增殖活性具有浓度依赖性;索拉菲尼还可明显增强p38MAPK的活化,而SB203580可显著缓解索拉菲尼诱导的TCA8113细胞活力下降。结论:索拉菲尼可抑制人口腔癌TCA8113细胞的增殖,其机制可能与其增强p38MAPK活化有关。
[Abstract]:Aim: to observe the effect of Solafini on the proliferation of human oral cancer TCA8113 cells and its mechanism. Methods: the proliferation of human oral cancer TCA8113 cells was detected by MTT assay and colony formation assay. The effects of different concentrations of Lafenil on the activation of p38MAPK were detected by Western blotting (Western blot) after 48 h intervention of TCA8113 cells with a concentration of 2.5 渭 g 路ml ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1). In order to detect the effect of p38MAPK on the proliferation of TCA8113 cells, 10 渭 mol/L SB203580(p38MAPK specific inhibitor was used to pretreat TCA81 13 cells for 30 min, then different concentrations of Solafenil were added to culture for 48 h, and the proliferation of TCA81 13 cells was detected by MTT assay. Results: Solafinil significantly inhibited the proliferation of TCA8113 cells in a concentration-dependent manner, and Solafini significantly enhanced the activation of p38MAPK, while SB203580 significantly alleviated the decrease in the activity of TCA8113 cells induced by Solafini. Conclusion: Solafinil can inhibit the proliferation of human oral cancer TCA8113 cells, and its mechanism may be related to its enhanced p38MAPK activation.
【作者单位】：锦州医科大学附属第一医院口腔科;潍坊市人民医院口腔颌面外科;锦州医科大学生理教研室;锦州医科大学护理学院;
【分类号】：R739.8

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