DCLK1、Bmi-1在大肠癌中的表达及临床意义

发布时间：2018-04-21 07:52

本文选题：大肠癌 + 双肾上腺素样激酶1(DCLK1)　；参考：《延安大学》2017年硕士论文

【摘要】：【目的】:本研究旨在联合检测DCLK1和Bmi-1在74例大肠癌组织中的表达状况,了解DCLK1和Bmi-1与大肠癌之间的关系。【方法】:收集延安大学附属医院2015年9月到2016年10月74例大肠癌患者完整的病理资料、临床资料,根治术前均未行放化疗及其他相关治疗,所选实验标本包括大肠癌及相对应的癌旁正常组织(注:正常组织距癌组织1cm,镜下未见癌细胞、腺瘤性增生细胞等异常细胞)。采用免疫组化Max Vision TM染色法检测DCLK1和Bmi-1在大肠癌中的表达状况,统计分析两者之间相关性及两者与大肠癌临床病理参数之间的关系。全部数据利用软件SPSS20.0进行统计学分析,计数资料采纳2x检验,DCLK1和Bmi-1的相关性采用Spearman等级相关分析,定义差异有统计学意义是P0.05,差异性显著则是P0.01。【结果】:1.DCLK1蛋白在大肠癌组织的阳性表达率(68.9%)明显超过癌旁正常组织(27.0%),黄色或棕黄色的染色免疫产物主要定位于细胞浆,呈颗粒状;经2x检验,2x=26.016,P0.01,差异性显著;与大肠癌浸润深度、淋巴结转移、Dukes分期存在显著相关性(P0.05),差异有统计学意义;与患者的性别、年龄、肿瘤部位、肿瘤分化程度、肿瘤分型均无显著相关性(P0.05)。2.Bmi-1蛋白在大肠癌组织的阳性表达率(62.2%)明显超过癌旁正常组织(23.0%),黄色、棕黄色或棕褐色的染色免疫产物主要定位在细胞核,少部分在细胞浆,呈颗粒状,经2x检验,2x=23.243,P0.01,差异性显著;与肿瘤分化程度、浸润深度、淋巴结转移、Dukes分期存在显著相关性(P0.05),差异有统计学意义;与患者的性别、年龄、肿瘤部位、肿瘤分型均无显著相关性(P0.05)。3.DCLK1与Bmi-1在大肠癌组织中表达水平之间的关系经Spearman等级相关分析后结果显示存在一定相关性,呈正相关(rs=0.259,P=0.026,P0.05),差异有统计学意义。【结论】:1.在大肠癌组织中DCLK1呈高表达,与大肠癌浸润、淋巴结转移、Dukes分期均存在显著相关性。2.在大肠癌组织中Bmi-1呈高表达,与大肠癌分化、浸润、淋巴结转移、Dukes分期均存在显著相关性。3.在大肠癌组织中DCLK1和Bmi-1的表达存在一定相关性,呈正相关,提示DCLK1可能通过某种机制间接影响Bmi-1的表达,两者可能协同参加大肠癌的发病、进展、浸润及转移;抑制两者的表达可能会阻断肿瘤的发生及发展;联合检测DCLK1和Bmi-1可能为大肠癌的临床诊治及预后评判提供新途径。
[Abstract]:[objective]: to investigate the expression of DCLK1 and Bmi-1 in 74 cases of colorectal carcinoma. To understand the relationship between DCLK1, Bmi-1 and colorectal cancer. [methods] 74 patients with colorectal cancer from September 2015 to October 2016 were collected from affiliated Hospital of Yan'an University. The selected specimens included colorectal cancer and the corresponding adjacent normal tissue (note: normal tissue from cancer tissue 1 cm, no cancer cells under the microscope, adenomatous hyperplasia cells and other abnormal cells. Immunohistochemical Max Vision TM staining was used to detect the expression of DCLK1 and Bmi-1 in colorectal carcinoma, and the correlation between them and clinicopathological parameters was analyzed statistically. All the data were statistically analyzed by software SPSS20.0, and the correlation between DCLK1 and Bmi-1 was analyzed by using 2x test. Spearman grade correlation analysis was used to analyze the correlation between DCLK1 and Bmi-1. The difference in definition was P0.05, and the difference was P0.01.Results the positive expression rate of DCLK1 protein in colorectal carcinoma was significantly higher than that in adjacent normal tissues (P 0.05 and P 0.01.Results) the positive expression rate of DCLK1 protein in colorectal cancer tissues was significantly higher than that in adjacent normal tissues. The immunoreactive products of yellow or brown staining were mainly located in cytoplasm. The difference was significant with the depth of invasion of colorectal cancer and the Dukes stage of lymph node metastasis, the difference was statistically significant, and the difference was statistically significant with the sex, age, tumor location and tumor differentiation of the patients. The positive expression rate of Bmi-1 protein in colorectal carcinoma tissues was significantly higher than that in adjacent normal tissues. The immunoreactive products of yellow, brown or brown staining were mainly located in the nucleus, a few in the cytoplasm, and were granular. By 2x test, the difference was significant, and there was significant correlation with tumor differentiation, depth of invasion and Dukes stage of lymph node metastasis (P 0.05), sex, age and location of tumor. There was no significant correlation between tumor classification and the expression level of Bmi-1 in colorectal cancer tissues. The results of Spearman grade correlation analysis showed that there was a positive correlation between P0.05T and Bmi-1 in colorectal carcinoma. [conclusion] there was a significant difference between two groups. [conclusion] there is a significant difference in the expression of Bmi-1 in colorectal carcinoma. [conclusion] the expression level of DCLK1 in colorectal carcinoma is significantly higher than that in control group. [conclusion] there is a significant difference in the expression of Bmi-1 in colorectal carcinoma. High expression of DCLK1 was found in colorectal carcinoma, which was significantly correlated with invasion of colorectal carcinoma, lymph node metastasis and Dukes stage. High expression of Bmi-1 was found in colorectal carcinoma, which was significantly correlated with differentiation, invasion, lymph node metastasis and Dukes stage of colorectal carcinoma. There was a positive correlation between DCLK1 and Bmi-1 expression in colorectal carcinoma, which suggested that DCLK1 might indirectly affect the expression of Bmi-1 through some mechanism, and they might co-participate in the pathogenesis, progression, invasion and metastasis of colorectal carcinoma. The combination of DCLK1 and Bmi-1 may provide a new approach for clinical diagnosis and treatment and prognosis evaluation of colorectal cancer.
【学位授予单位】：延安大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.34

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