人参皂苷Rg5对人食管癌细胞作用的研究

发布时间：2018-04-22 21:27

本文选题：人参皂苷Rg + 食管癌细胞Eca　；参考：《中国实验诊断学》2016年12期

【摘要】：目的探索人参皂苷Rg5对人食管癌细胞Eca-109的作用及机制。方法利用不同浓度人参皂苷Rg5在体外对人食管癌Eca109细胞作用不同时间,采用CCK-8法检测细胞抑制率,Annexin V-FITC/PI双染色后流式细胞仪检测细胞凋亡率,PI单染法检测细胞周期。结果在浓度分别为50、100μmol/L的人参皂苷Rg5作用人食管癌细胞24h后出现明显形态学变化,同时处于S期及G2/M期细胞较对照组明显增多,分别为53.08%、25.84%和58.44%、31.48%,流式细胞仪检测早期凋亡率分别为11.45%、30.13%。结论人参皂苷Rg5能抑制食管癌Eca-109细胞增殖,其机制可能为增加细胞早期凋亡,引起细胞周期滞留。
[Abstract]:Objective to investigate the effect and mechanism of ginsenoside Rg5 on human esophageal carcinoma cell line Eca-109. Methods different concentrations of ginsenoside Rg5 were used to treat human esophageal carcinoma Eca109 cells for different time in vitro. The inhibition rate of human esophageal carcinoma Eca109 cells was detected by CCK-8 assay and the apoptosis rate was detected by flow cytometry and the cell cycle was detected by Pi single staining after Annexin V-FITC/PI double staining. Results after treated with ginsenoside Rg5 at the concentration of 50100 渭 mol/L for 24 hours, there were obvious morphological changes in human esophageal carcinoma cells, and the number of cells in S phase and G 2 / M phase was significantly higher than that in control group (53.08% and 58.4444%, 31.48%, respectively). The early apoptotic rate was 11.45% by flow cytometry. Conclusion ginsenoside Rg5 can inhibit the proliferation of esophageal carcinoma Eca-109 cells by increasing early apoptosis and cell cycle retention.
【作者单位】：吉林大学第二医院;吉林大学基础医学院;吉林大学生命科学院;
【基金】：国家自然科学基金重大国际合作项目(项目编号:81320108025)
【分类号】：R285

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