槲皮素PLGA-TPGS纳米粒对小鼠肝癌异位实体瘤治疗效果的研究

发布时间：2018-04-22 22:39

  本文选题：槲皮素 + 纳米粒　； 参考：《中国医科大学学报》2017年09期

【摘要】：目的考察槲皮素PLGA-TPGS纳米粒(QPTN)在体内对荷腹水型肝癌高淋巴道转移细胞HCa-F小鼠异位移植实体瘤的治疗效果。方法建立荷HCa-F肝癌细胞小鼠模型后,随机分为阴性对照组、空白纳米粒组、5-氟尿嘧啶溶液(FS)组、槲皮素溶液(QTS)组、槲皮素PLGA纳米粒(QPN)组和QPTN组。尾静脉给药,每2 d 1次,连续给药20 d后处死小鼠,剥离肿瘤,称质量,测量肿瘤体积,根据公式计算肿瘤体积增长量和抑瘤率;行HE染色观察肿瘤,全面评价QPTN对荷瘤小鼠的治疗效果。结果小鼠体内给药10次后,QPTN组、QPN组、FS组的肿瘤体积增长量与阴性对照组相比明显减小(P0.05或P0.01),QPTN组抑瘤率(59.07%)明显高于QTS组(23.94%)、FS组(35.14%)和QPN组(46.14%)。HE染色结果也显示QPTN组对小鼠肿瘤的治疗效果最明显。结论与QPN、QTS和FS相比,QPTN对荷HCa-F肝癌细胞小鼠异位实体瘤具有较好的治疗效果。
[Abstract]:Objective to investigate the therapeutic effect of quercetin PLGA-TPGS nanoparticles in vivo on xenografted solid tumor in HCa-F mice with high lymphatic metastasis cells of ascites hepatoma. Methods mice bearing HCa-F hepatoma cells were randomly divided into three groups: negative control group, blank nanoparticles group, quercetin solution group, quercetin PLGA nanoparticles group and QPTN group. The mice were sacrificed every 2 days, 20 days later, the tumor was stripped, the tumor was weighed, the tumor volume was measured, the tumor volume growth and tumor inhibition rate were calculated according to the formula, and the tumor was observed by HE staining. To evaluate the therapeutic effect of QPTN on tumor-bearing mice. Results after 10 times of administration, the tumor volume increase in the FS group in the QPTN group was significantly lower than that in the negative control group (P 0.05 or P 0.01). The tumor inhibition rate in the QPTN group was significantly higher than that in the QTS group (P < 0.05) and in the QPN group (46.14.HE staining). The therapeutic effect of tumor is the most obvious. Conclusion compared with QPN- QTS and FS, QPTN has a better therapeutic effect on heterotopic solid tumor in mice bearing HCa-F hepatoma cells.
【作者单位】： 大连医科大学基础医学院机能学实验室;大连医科大学药学院药剂学教研室;大连医科大学;
【基金】：辽宁省自然科学基金(2015020308)
【分类号】：R735.7
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本文编号：1789287