鲟鱼硫酸软骨素对结直肠癌细胞抑制作用

发布时间：2018-04-24 18:59

本文选题：鲟鱼硫酸软骨素 + 结直肠癌　；参考：《食品科学》2017年21期

【摘要】：探究鲟鱼硫酸软骨素对结直肠癌细胞的生长抑制能力,并初步确定影响结直肠癌细胞生长的主要原因。选取3株具有代表性的结直肠癌细胞Caco-2、HCT-116、SW480,采用CCK-8法测定细胞生长曲线、硫酸软骨素对癌细胞增殖的抑制能力;以其中一株结直肠癌细胞HCT-116为模型,用流式细胞仪测定其周期和凋亡情况,用细胞核染色法观察处理前后细胞形态,结合caspase-3凋亡酶活力的测定,考察鲟鱼硫酸软骨素对结直肠癌细胞的促凋亡能力,并初步确定作用途径。研究结果表明,鲟鱼硫酸软骨素对结直肠癌细胞Caco-2、HCT-116、SW480的增殖具有一定抑制作用,最高抑制率分别为70.94%、90.00%和75.00%,明显高于阳性对照处理组;处理后,细胞周期G1/G0期比例升高至88.56%,S期比例降低至4.47%,阻滞细胞G1/G0期;同时,使用鲟鱼硫酸软骨素处理细胞后,细胞形态发生变化,出现细胞核固缩以及细胞破碎等现象,细胞主要的凋亡酶活力显著升高,酶活力可达1 645 IU/μg,使细胞发生凋亡,凋亡率可达63.73%。研究结果证明,鲟鱼硫酸软骨素具有促进结直肠癌细胞凋亡、抑制细胞增殖的能力。
[Abstract]:To investigate the inhibitory effect of chondroitin sulfate on the growth of colorectal cancer cells, and to determine the main reasons that affect the growth of colorectal cancer cells. Three representative colorectal cancer cell lines, Caco-2HCT-116C480, were selected to determine the cell growth curve by CCK-8 assay and the inhibitory effect of chondroitin sulfate on the proliferation of cancer cells, one of which was used as a model for colorectal cancer cell line HCT-116. Cell cycle and apoptosis were measured by flow cytometry, cell morphology was observed by nuclear staining before and after treatment, and the apoptotic ability of Sturgeon chondroitin sulfate on colorectal cancer cells was studied by combining the activity of caspase-3 apoptotic enzyme. The action path was preliminarily determined. The results showed that chondroitin sulfate of Sturgeon had a certain inhibitory effect on the proliferation of colorectal cancer cell line Caco-2HCT-116 and SW480, and the highest inhibitory rates were 70.944% and 75.00%, respectively, which were significantly higher than those in the positive control group. The proportion of G1/G0 phase in cell cycle increased to 88.56 and the proportion of S phase decreased to 4.47%, which blocked the cell G1/G0 phase. At the same time, after treated with chondroitin sulfate from Sturgeon, the cell morphology changed, cell nucleus pyknosis and cell fragmentation occurred. The activity of the main apoptotic enzyme increased significantly, and the activity of the main apoptotic enzyme reached 1 645 IU/ 渭 g. The apoptotic rate was 63.73%. The results showed that chondroitin sulfate from Sturgeon had the ability of promoting apoptosis and inhibiting cell proliferation.
【作者单位】：内蒙古大学生命科学学院;中国农业大学食品科学与营养工程学院;
【基金】：北京市鲟鱼、鲑鳟鱼创新团队项目(SCGWZJ201711)
【分类号】：R73-36

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