基于超声波穿孔效应的细胞SERS光谱研究

发布时间：2018-04-27 13:00

本文选题：超声波穿孔效应 + 金属纳米颗粒　；参考：《福建师范大学》2015年硕士论文

【摘要】：本论文阐述了超声波穿孔效应的细胞SERS光谱研究。针对现有将金属纳米粒子导入活细胞中方法上存在的缺陷,我们首次开展了超声波穿孔效应-SERS光谱技术研究,即通过超声波穿孔效应将金属纳米颗粒(金和银)快速(1分钟)导入活细胞中作为SERS增强基底,获得细胞的SERS光谱,从而实现对细胞内各物质生化成分的研究及多种癌细胞的检测和筛查应用。主要研究成果如下：1、利用超声波穿孔效应将银纳米颗粒快速导入K562、NP69及C666细胞,结合超声波穿孔后的细胞TEM图像验证了超声波穿孔效应确实能够使得金属纳米颗粒快速进入细胞内；2、通过细胞超声波穿孔效应-SERS光谱的分析获得了最佳的优化测试条件：采用20倍物镜并将光斑扩束到100%；3、利用超声波穿孔效应法与传统孵育内吞法下获得全细胞SERS光谱存在很大差异,可能跟银纳米颗粒具有毒性作用有关；4、通过超声波穿孔效应-SERS技术获得的正常鼻咽NP69细胞与鼻咽癌C666细胞的SERS光谱,从而进行癌症细胞的筛查研究；其次,用同样方法对比分析NP69与K562细胞；最后再进行三种细胞的区分筛查与分析。实验结果表明,超声波穿孔效应-SERS技术方法可以在1分钟内将金和银纳米颗粒快速导入活细胞内部并作为SERS增强基底,通过检测细胞SERS光谱,可快速确定细胞的生化成分,并应用于癌细胞的筛查研究。该方法不仅具有操作简便快速、分辨率高等优点,而且扩展了SERS技术在细胞生化研究领域的应用,其应用前景十分广阔。
[Abstract]:In this paper, the cell SERS spectroscopy of ultrasonic perforation is described. In view of the defects of the existing methods for the introduction of metal nanoparticles into living cells, we have carried out the first research on ultrasonic perforation effect (SERS) spectroscopy. In other words, the metal nanoparticles (gold and silver) were introduced into living cells as SERS enhancement substrate by ultrasonic piercing effect for 1 minute, and the SERS spectra of the cells were obtained. Thus, the biochemical components of various substances in the cell and the detection and screening of many kinds of cancer cells can be realized. The main results are as follows: 1. Silver nanoparticles were rapidly introduced into K562NP-69 and C666 cells by ultrasonic perforation. Combined with the TEM images of cells after ultrasonic perforation, it was proved that the ultrasonic perforation effect could make metal nanoparticles enter the cell rapidly. The best optimized measurement was obtained by the analysis of cell ultrasonic perforation effect -SERS spectra. The test conditions were as follows: using 20 times objective lens and expanding the light spot to 100 ~ 3, the SERS spectra of whole cell obtained by ultrasonic perforation method and traditional incubation endocytosis method were very different. It may be related to the toxic effect of silver nanoparticles. The SERS spectra of normal nasopharyngeal NP69 cells and nasopharyngeal carcinoma C666 cells obtained by ultrasonic perforation effect -SERS technique were used to screen cancer cells. NP69 and K562 cells were compared with each other by the same method. The experimental results show that the ultrasonic perforation effect -SERS technique can quickly transfer gold and silver nanoparticles into living cells within one minute and act as a SERS enhancement substrate. The biochemical components of the cells can be quickly determined by detecting the SERS spectra of the cells. And applied to the screening of cancer cells. This method not only has the advantages of simple and fast operation and high resolution, but also extends the application of SERS technology in the field of cell biochemistry, and its application prospect is very broad.
【学位授予单位】：福建师范大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R730.4;O657.3

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