ENO1在HBV相关肝癌中的表达及生物学意义

发布时间：2018-04-28 20:37

本文选题：ENO1 + HBV　；参考：《宁夏医科大学》2017年硕士论文

【摘要】：目的:探讨ENO1在HBV相关原发性肝癌中的表达及其对肝癌细胞生物学活性的影响及可能机制。方法:利用q RT-PCR、Western blot、ELISA技术对ENO1在肝癌及癌旁组织、肝癌细胞及正常肝细胞、血清中的表达进行差异性分析。采用si RNA干扰技术对Hep G2细胞的ENO1基因进行敲减,然后利用CCK-8、克隆形成、transwell实验技术检测Hep G2细胞增殖、迁移、侵袭能力的变化,实时定量PCR检测Notch信号通路下游靶基因HEY1,HES1,HES5的差异性表达,Western blot技术检测NICD及EMT相关蛋白snail、slug、β-Catenin,E-Cadherin和Vimentin的差异性表达。结果:ENO1在HBV相关肝癌组织和Hep G2.215细胞系中明显高表达。下调ENO1的表达明显降低肝癌细胞的增殖、克隆形成、迁移、侵袭能力。进一步的机制研究表明下调ENO1的表达明显降低HEY1,HES1,HES5基因和NICD,snail,slug蛋白的表达,然而β-Catenin,E-Cadherin和Vimentin的表达并未发生明显改变。结论:ENO1与肝细胞肝癌的发生发展有关,并与HBV感染密切相关,ENO1可能通过Notch信号通路调控肝细胞肝癌的生物学活性。
[Abstract]:Aim: to investigate the expression of ENO1 in HBV associated primary hepatocellular carcinoma (PHC) and its effect on the biological activity of HCC cells and its possible mechanism. Methods: the expression of ENO1 in liver cancer and adjacent tissues, hepatoma cells and normal hepatocytes and serum were analyzed by Q RT-PCR- Western blotl Elisa. Si RNA interference technique was used to knockout the ENO1 gene in Hep G2 cells, and then CCK-8 was used to detect the proliferation, migration and invasion ability of Hep G2 cells. The differential expression of the downstream target gene HEY1, HES1, HES5 in Notch signaling pathway was detected by real-time quantitative PCR. Western blot technique was used to detect the differential expression of NICD and EMT related proteins snailslug, 尾 -Catenin E-Cadherin and Vimentin. Results the expression of ENO1 was significantly higher in HBV associated liver cancer tissues and Hep G2.215 cell lines. Down-regulation of ENO1 expression significantly reduced the proliferation, clone formation, migration and invasion of hepatoma cells. Further studies showed that down-regulation of the expression of ENO1 significantly decreased the expression of HEY1, HES1, HES5 and NICDX snailslug protein, but the expression of 尾 -Catenin E-Cadherin and Vimentin did not change significantly. ConclusionENO1 is related to the occurrence and development of hepatocellular carcinoma, and closely related to HBV infection. ENO1 may regulate the biological activity of HCC through Notch signaling pathway.
【学位授予单位】：宁夏医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.7;R512.62

【参考文献】