肝癌组织长链非编码RNA RP13-143G15.3表达临床意义分析

发布时间：2018-04-29 15:54

  本文选题：长链非编码RNA + 肝癌　； 参考：《中华肿瘤防治杂志》2017年12期

【摘要】：目的近几年,大量的长链非编码RNA被发现,通过参与表观遗传、转录和翻译等实现对基因表达的调控,促进肿瘤细胞的发生发展和转移。探究长链非编码RNA RP13-143G15.3在肝癌及癌旁组织中的表达水平,分析其差异表达与临床指标之间的相关性,为肝癌的诊疗提供新的标志物。方法对5例肝癌及癌旁组织进行长链非编码RNA(lncRNAs)表达谱筛查,选择具有表达差异的RP13-143G15.3扩大样本进行验证。收集2016-03-01-2016-06-30广西医科大学肿瘤医院肝胆外科进行肝癌切除术的56例患者肝癌及癌旁组织,采用实时荧光定量PCR技术检测RP13-143G15.3的相对表达水平,并分析其差异表达与患者临床特征、血清学指标及免疫组化指标的相关性。结果5例肝癌组织中RP13-143G15.3表达高于癌旁组织,P=0.005。在大样本56例肝癌组织中相对表达量为0.026 5±0.006,癌旁组织中相对表达量为0.040 7±0.025,肝癌组织中RP13-143G15.3表达明显低于相应癌旁组织(Z=-3.842,P0.001),与芯片筛查结果不一致。RP13-143G15.3的差异表达与患者乙肝携带史(χ~2=4.667,P=0.031)、门脉高压(χ~2=5.250,P=0.022)、甲胎蛋白水平(χ~2=4.791,P=0.029)、乳酸脱氢酶(Z=-2.237,P=0.025)、谷氨酸脱氢酶(Z=-2.101,P=0.036)、纤维蛋白原(t=-2.103,P=0.040)、CA72-4(Z=-2.147,P=0.032)、免疫学指标CD34(χ~2=9.516,P=0.023)及肿瘤增殖抗原(χ~2=8.000,P=0.005)有关。结论 RP13-143G15.3在肝癌组织中表达下调,其可能起到抑癌作用,有望成为肝癌新的肿瘤标志物及分子靶点,为后续功能研究及机制研究提供理论基础。
[Abstract]:Objective in recent years, a large number of long chain noncoding RNA have been found to regulate gene expression by participating in epigenetics, transcription and translation, and to promote the development and metastasis of tumor cells. To explore the expression level of long chain noncoding RNA RP13-143G15.3 in hepatocellular carcinoma and its adjacent tissues, and to analyze the correlation between the differential expression and clinical indexes, and to provide a new marker for the diagnosis and treatment of liver cancer. Methods 5 cases of liver cancer and its adjacent tissues were screened for long chain noncoding RNAs lncRNAs. the expanded RP13-143G15.3 samples with different expression were selected for verification. The relative expression of RP13-143G15.3 was detected by real-time fluorescence quantitative PCR in 56 patients undergoing hepatectomy by hepatobiliary surgery, Cancer Hospital, Guangxi Medical University, 2016-03-01-2016-06-30. The differential expression and clinical features of HCC were analyzed. Correlation of serological and immunohistochemical indexes. Results the expression of RP13-143G15.3 in 5 HCC tissues was higher than that in the adjacent tissues. The relative expression of RP13-143G15.3 was not consistent with the results of microarray screening. The relative expression of RP13-143G15.3 was 0.026 卤0.006 in 56 HCC tissues and 0.040 7 卤0.025 in paracancerous tissues. The expression of RP13-143G15.3 in HCC tissues was significantly lower than that in the corresponding adjacent tissues. The difference between the expression of RP13-143G15.3 and the results of microarray screening was not consistent with the results of microarray screening. 涔欒倽鎼哄甫鍙，

本文编号：1820595