微小RNA-23a对乳腺癌细胞增殖和侵袭的影响

发布时间：2018-04-29 17:23

  本文选题：乳腺癌 + 微小RNA-a　； 参考：《中国临床药理学杂志》2017年16期

【摘要】：目的研究微小RNA-23a(miR-23a)对乳腺癌细胞增殖和侵袭的影响。方法将人乳腺癌MCF-7和MDA-MB-435S复苏培养后,接种至6孔板中,随机分组进行培养,共分为6组:RNAi-Ⅰ组、过表达-Ⅰ组、对照-Ⅰ组、RNAi-Ⅱ组、过表达-Ⅱ组、对照-Ⅱ组。构建MiR-23a重组干扰及过表达载体,用Lipofectamine 2000将构建成功的MiR-23a重组干扰及过表达载体分别转染至各组人乳腺癌MCF-7和MDA-MB-435S细胞系中。结果在培养72 h后的OD492值:过表达组的MCF-7细胞和MDA-MB-435S细胞可分别达到0.52±0.03和0.44±0.03,而RNAi组MCF-7细胞和MDA-MB-435S细胞仅为0.36±0.02和0.31±0.02,与对照-Ⅰ组和对照-Ⅱ组比较,RNAi-Ⅰ组和RNAi-Ⅱ组的差异均有统计学意义(均P0.05)。过表达-Ⅰ组和过表达-Ⅱ组的细胞倍增时间分别为(26.61±5.23),(29.23±5.51)h,而RNAi-Ⅰ组和RNAi-Ⅱ组的细胞则分别为(42.24±3.47),(58.17±5.34)h。过表达-Ⅰ组和过表达-Ⅱ组的M期细胞比例分别为52.36%,51.59%;而RNAi-Ⅰ组和RNAi-Ⅱ组则分别为76.70%,74.17%。与对照-Ⅰ组比较,RNAi-Ⅰ组和过表达-Ⅰ组的细胞倍增时间及M期比例差异均有统计学意义(均P0.05)。结论 MiR-23a的表达水平与乳腺癌细胞的增殖和侵袭呈现正相关性。
[Abstract]:Objective to study the effect of microRNA-23a- miR-23a on the proliferation and invasion of breast cancer cells. Methods Human breast cancer MCF-7 and MDA-MB-435S were resuscitated and inoculated into 6-well plate, and were divided into 6 groups randomly. They were divided into 6 groups: group 鈪，

本文编号：1820869