GRP78、CHOP在α-细辛醚诱导的Eca109细胞凋亡中的作用

发布时间：2018-04-29 17:57

本文选题：α-细辛醚 + Eca细胞　；参考：《郑州大学学报(医学版)》2017年02期

【摘要】：目的:研究葡萄糖调节蛋白78(GRP78)和C/EBP环磷酸腺苷反应元件结合转录因子同源蛋白(CHOP)在α-细辛醚诱导的人食管癌Eca109细胞凋亡中的作用。方法:采用0.25、0.50及1.00 g/L的α-细辛醚作用于体外培养的Eca109细胞,并以无干预的细胞作为对照。分别于培养后12、24、36、48 h,采用MTT法检测Eca109细胞的增殖抑制情况;采用免疫荧光染色法检测细胞凋亡情况;运用Real-time PCR和Western blot检测凋亡相关基因GRP78和CHOP的表达水平。结果:α-细辛醚对Eca109细胞的增殖具有抑制作用(P0.05),且具有时间、剂量效应关系。α-细辛醚作用48 h后,GRP78和CHOP mRNA和蛋白的相对表达量明显增加(P0.05)。结论:α-细辛醚可抑制Eca109细胞增殖和诱导细胞凋亡,其机制与调节内质网应激相关蛋白GRP78、CHOP表达有关。
[Abstract]:Aim: to investigate the role of glucose-regulated protein 78 (GRP78) and C/EBP cyclic adenosine monophosphate binding transcription factor homologous protein (CHOPP) in apoptosis of human esophageal carcinoma Eca109 cells induced by 伪 -asarone. Methods: Eca109 cells were treated with 0.25g / L and 1.00g / L 伪 -Asarone in vitro, and non-intervention cells were used as control. The proliferation inhibition of Eca109 cells was detected by MTT assay, apoptosis was detected by immunofluorescence staining, and the expression levels of apoptosis-related genes GRP78 and CHOP were detected by Real-time PCR and Western blot. Results: 伪 -Asarone inhibited the proliferation of Eca109 cells in a time-dose and dose-dependent manner. After 48 h of treatment with 伪 -Asarone, the relative expression of GRP78, CHOP mRNA and protein increased significantly (P 0.05). Conclusion: 伪 -Asarone can inhibit the proliferation and induce apoptosis of Eca109 cells, and its mechanism is related to the regulation of the expression of endoplasmic reticulum stress-related protein GRP78 / chop.
【作者单位】：河南中医药大学基础医学院实验教学中心;
【基金】：郑州市科技攻关项目131PPTGG417-1 郑州市科技创新团队项目121PCXTD520
【分类号】：R735.1

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