血清DCD、CA199、CA724和CEA联合检测对大肠癌的诊断价值

发布时间：2018-05-01 02:14

本文选题：大肠癌 + DCD　；参考：《实用医学杂志》2017年15期

【摘要】：目的探究联合检测皮离蛋白(DCD)、糖类抗原199(CA199)、糖类抗原724(CA724)与癌胚抗原(CEA)肿瘤标志物对大肠癌早期诊断的可能性。方法 81例明确诊断的大肠癌患者作为病例组,同期纳入30例健康体检者作为对照组,采用ELISA方法检测血清中DCD的表达情况,并采用电化学发光免疫分析法检测血清中CEA、CA199、CA724的水平,采用Western blot检测DCD在大肠癌组织及癌旁组织中的表达。结果病例组血清中CA199、CA724以及CEA的浓度明显高于对照组,差异有统计学意义(P0.001);诊断大肠癌的ROC曲线下面积:CA199、CA724、CEA联合诊断为0.930,DCD、CA199、CA724、CEA联合诊断是0.942,均高于单独使用时的曲线下面积;病例组TNM分期越高,血清中CA199、CEA的表达量越高,且差异有统计学意义(P0.001),DCD在大肠癌组织中的表达浓度明显高于癌旁组织,差异有统计学意义(P0.05)。结论大肠癌组织DCD表达水平明显高于癌旁组织,血清中DCD、CA199、CA724以及CEA的表达有可能作为肿瘤联合诊断的标志物,对大肠癌的辅助诊断有较高的临床应用价值。
[Abstract]:Objective to explore the possibility of early diagnosis of colorectal cancer by combined detection of the tumor markers of dermatocytoprotein, carbohydrate antigen 1999, carbohydrate antigen 724 CA724 and carcinoembryonic antigen (CEA). Methods 81 patients with colorectal cancer were selected as case group and 30 healthy persons as control group. ELISA method was used to detect the expression of DCD in serum. Electrochemiluminescence immunoassay (ECLIA) was used to detect the level of CA199CA724 in serum, and Western blot was used to detect the expression of DCD in colorectal carcinoma and adjacent tissues. Results the serum levels of CA199, CA724 and CEA in patients with colorectal cancer were significantly higher than those in the control group (P 0.001), and the area under the ROC curve of diagnosis of colorectal cancer was 0.930 DCDCA199CA724 + CEA combined diagnosis was 0.942, which was higher than the area under the curve of single use. The higher the stage of TNM was, the higher the expression of CA199D was in serum, and the difference was statistically significant (P 0.001) in colorectal carcinoma tissues than in adjacent tissues (P 0.05). Conclusion the expression of DCD in colorectal carcinoma tissues is significantly higher than that in adjacent tissues. The expression of DCDCA199CA724 and CEA in serum may be used as a marker for the combined diagnosis of colorectal cancer and has a high clinical value in the auxiliary diagnosis of colorectal cancer.
【作者单位】：广州医科大学附属肿瘤医院检验科;广州市中医医院检验科;
【基金】：国家自然科学基金委员会-青年科学基金项目(编号:81503424) 广东省医学科学技术研究基金项目(编号:A2016552) 广东省自然科学基金项目(编号:2014A030313802) 广州市科创委协同创新重大专项(编号:201704020171)
【分类号】：R735.34

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