DNMT1和CCNA1在不同级别宫颈病变组织中的表达及临床意义

发布时间：2018-05-01 02:23

本文选题：DNA甲基转移酶 + 细胞周期蛋白A　；参考：《实用医学杂志》2017年24期

【摘要】：目的探讨DNMT1和CCNA1在不同级别宫颈病变组织中的表达及临床意义。方法选取病理学诊断的正常宫颈(NC)、宫颈低级别上皮内病变(LSIL)、宫颈高级别上皮内病变(HSIL)、宫颈鳞状细胞癌(SCC)组织各30例,采用Western blot法、实时荧光聚合酶链反应(q RT-PCR)法检测各组DNMT1、CCNA1 mRNA和蛋白的表达。结果 DNMT1 mRNA和蛋白表达量在LSIL、HSIL及SCC组织中均高于NC组织(F=117.93,P0.05;F=61.24,P0.05),随着病变程度的增加,DNMT1 mRNA和蛋白的表达水平逐渐上升(χ~2趋势=26.25,P0.05;χ~2趋势=26.60,P0.05)。CCNA1 mRNA和蛋白的表达水平在HSIL、SCC组织中均低于NC、LSIL组织(F=77.04,P0.05;F=57.15,P0.05),随着病变程度的增加,CCNA1 mRNA和蛋白表达量逐渐下降(χ~2趋势=64.19,P0.05;χ~2趋势=60.24,P0.05)。DNMT1、CCNA1 mRNA和蛋白在LSIL、HSIL、SCC组织中的表达呈负相关(r=-0.75、-0.56,P0.05)。结论 DNMT1 mRNA、蛋白的高表达,CCNA1 mRNA、蛋白低表达,二者可能与宫颈癌的发生发展有关。
[Abstract]:Objective to investigate the expression and clinical significance of DNMT1 and CCNA1 in cervical lesions of different grades. Methods 30 cases of normal cervical carcinoma, 30 cases of low grade intraepithelial lesion of cervix, 30 cases of high grade intraepithelial lesion of cervix and 30 cases of squamous cell carcinoma of cervix were selected. The expression of DNMT1CCNA1 mRNA and protein was detected by real-time fluorescence polymerase chain reaction (RT-PCR). Results the expression levels of DNMT1 mRNA and protein in HSIL and SCC tissues were higher than those in NC tissues. The expression levels of DNMT1 mRNA and protein gradually increased with the increase of pathological degree (蠂 2 trend was 26.25 P0.05; 蠂 2 trend was 26.60% P 0.05N. CCNA1 mRNA and protein level in HSILSCC tissues. The expression of CCNA1 mRNA and protein decreased gradually with the increase of pathological degree (蠂 ~ 2 trend: 64.19 P 0.05; 蠂 ~ 2 trend: 60.24P 0.05.DNMT1CCNA1 mRNA and protein were negatively correlated with the expression of CCNA1 mRNA and protein in LSILHSILSCC). Conclusion DNMT1 mRNAs, the high expression of CCNA1 mRNAs and the low expression of CCNA1 mRNAs, may be related to the occurrence and development of cervical cancer.
【作者单位】：华北理工大学基础医学院、河北省慢性疾病重点实验室、唐山市慢性病临床基础研究重点实验室;华北理工大学附属医院妇产科;
【基金】：河北省医学科学研究重点课题计划项目(编号:20160726)
【分类号】：R737.33

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