盐霉素联合顺铂对人胃癌细胞MKN-45增殖和凋亡的影响

发布时间：2018-05-01 08:06

本文选题：盐霉素 + 顺铂　；参考：《山东大学学报(医学版)》2017年05期

【摘要】：目的观察盐霉素联合顺铂对人胃癌细胞MKN-45增殖和凋亡的影响并探讨其作用机制。方法体外培养人胃癌细胞MKN-45至对数生长期,采用噻唑蓝(MTT)比色法分别检测盐霉素、顺铂及联合用药时细胞的增殖情况;吖啶橙/溴化乙锭(AO/EB)凋亡试剂盒荧光染色观察细胞凋亡情况;Western blotting法检测NF-κB、caspase-3蛋白表达水平。结果 MTT法显示盐霉素、顺铂均可抑制细胞增殖(P0.05),联合给药比单药抑制效果更为显著(P0.05);AO/EB荧光染色可见盐霉素、顺铂分别单药给药后,与对照组相比细胞凋亡形态明显,两药联合凋亡效果更为显著;Western blotting结果显示联合组与单药组和对照组相比NF-κB蛋白表达下降(P0.01),caspase-3蛋白表达升高(P0.01)。结论盐霉素可抑制胃癌细胞增殖,与顺铂联合应用可增强顺铂对人胃癌细胞MKN-45的抑制作用,其机制可能为抑制NF-κB活化,上调下游凋亡基因caspase-3的表达。
[Abstract]:Objective to investigate the effect of salinomycin combined with cisplatin on proliferation and apoptosis of human gastric cancer cell line MKN-45. Methods Human gastric cancer cells were cultured from MKN-45 to logarithmic growth stage in vitro. The proliferation of human gastric cancer cells was detected by thiazolyl methadone colorimetry and the combination of salinomycin and cisplatin. Acridine orange / ethidium bromide AOP / EBB apoptosis kit was used to detect apoptosis by fluorescence staining. Western blotting assay was used to detect the expression of NF- 魏 B caspase-3 protein. Results MTT assay showed that both salinomycin and cisplatin could inhibit cell proliferation. The combined administration of salinomycin and cisplatin was more effective than single drug in inhibiting cell proliferation. The results of Western blotting showed that the expression of NF- 魏 B protein in the combined group was significantly lower than that in the single drug group and the control group. Conclusion Salinomycin can inhibit the proliferation of gastric cancer cells, and the combination of cisplatin and cisplatin can enhance the inhibitory effect of cisplatin on human gastric cancer cell line MKN-45. The mechanism may be to inhibit the activation of NF- 魏 B and up-regulate the expression of downstream apoptotic gene caspase-3.
【作者单位】：延安大学医学院;延安市肿瘤防治研究重点研究室;延安大学附属医院检验科;
【基金】：国家大学生创新创业项目(2015 NO16956) 延安大学研究生教育创新项目(YCX201637);延安大学校级青年项目(YDQ2016-30)
【分类号】：R735.2

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