多柔比星对乳腺癌细胞核内丝切蛋白相互作用蛋白影响的比较蛋白质组学研究

发布时间：2018-05-04 08:21

本文选题：多柔比星 + 乳腺癌　；参考：《第三军医大学学报》2017年06期

【摘要】：目的采用比较蛋白质组学的方法,检测多柔比星对乳腺癌细胞核内丝切蛋白(cofilin)相互作用蛋白的影响。方法采用Western blot检测多柔比星对细胞核内cofilin表达的影响;免疫共沉淀后,采用比较蛋白质组学方法,检测对照组与多柔比星处理组细胞核内cofilin相互作用蛋白的差异;采用流式细胞仪检测多柔比星对细胞周期的影响。结果多柔比星处理MDA-MB-231细胞后,细胞核内cofilin表达增加,呈明显量效和时效关系;与对照组比较,多柔比星处理组核内与cofilin相互作用的蛋白结合蛋白、结构分子激活蛋白、细胞骨架蛋白、核酸结合蛋白、核糖体蛋白的种类明显减少(109、60、45、76、34 vs 79、32、20、56、16);多柔比星处理细胞后,可引起细胞周期阻滞在S/G2期,并呈明显量效关系,G1期细胞由(45.92±4.57)%降至(10.50±0.83)%(P0.01)。结论多柔比星处理细胞后可增加cofilin核聚集,减少细胞核内与cofilin相互作用蛋白的种类,最终使细胞周期阻滞在S/G2期。
[Abstract]:Objective to detect the effect of doxorubicin on the interacted protein of silkcut protein cofilin in the nucleus of breast cancer by a comparative proteomics method. Methods the effect of doxorubicin on the expression of cofilin in nucleus was detected by Western blot, and the difference of cofilin interaction protein between control group and doxorubicin treated group was detected by comparative proteomics after co-immunoprecipitation. The effect of doxorubicin on cell cycle was detected by flow cytometry. Results after treated with doxorubicin, the expression of cofilin in the nucleus of MDA-MB-231 cells increased significantly in dose-effect and time-dependent manner, and compared with the control group, the protein binding protein and structural molecular activator protein interacting with cofilin in the nucleus of doxorubicin treated group were significantly increased. The types of cytoskeleton protein, nucleic acid binding protein and ribosomal protein decreased significantly (P 0.01) after treated with doxorubicin from 45.92 卤4.57% to 10.50 卤0.83P0.01. after treated with doxorubicin, the cell cycle was blocked in S/G2 phase. Conclusion doxorubicin can increase the nuclear aggregation of cofilin, decrease the type of interacting protein with cofilin in the nucleus, and finally block the cell cycle in S/G2 phase after treated with doxorubicin.
【作者单位】：第三军医大学药学系生药学与天然药物化学教研室;
【基金】：国家自然科学基金面上项目(31571425)~~
【分类号】：R737.9

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