CIK细胞对LLC小鼠肺癌细胞株诱导凋亡的影响

发布时间：2018-05-07 06:57

本文选题：CIK细胞 + LLC小鼠肺癌细胞株　；参考：《中国老年学杂志》2017年22期

【摘要】：目的探讨干扰素(IFN)-γ、白细胞介素(IL)-2及CD3抗体诱导细胞因子诱导的杀伤细胞(CIK)对LLC小鼠肺癌细胞株存活率和凋亡相关因子14-3-3ζ及p-Bad蛋白表达的影响。方法常规CIK细胞的体外培养方法培养CIK细胞为效应细胞,LLC小鼠肺癌细胞株为靶细胞,通过复合培养体系(Transwell培养板)将CIK细胞与LLC小鼠肺癌细胞株建立共培养体系。根据培养时间不同分3组(10、15、20 d)运用四甲基唑蓝(MTT)比色法检测LLC小鼠肺癌细胞的存活率。Western印迹检测CIK细胞14-3-3ζ、p-Bad蛋白表达变化。结果光镜下观察发现,成功地诱导出CIK细胞。培养20 d时LLC肺癌细胞的存活率明显低于10 d组和15 d组(P0.01)。Western印迹检测LLC小鼠肺癌细胞中14-3-3ζ、p-Bad的表达均有下调。结论随着培养时间的延长CIK细胞对LLC小鼠肺癌细胞的存活率逐渐降低并能促进LLC小鼠肺癌细胞的凋亡。
[Abstract]:Objective to investigate the effects of IFN- 纬, IL-IL-2and CD3 antibody on the survival rate, apoptosis related factor 14-3-3 味 and the expression of p-Bad protein in LLC mice lung cancer cell lines. Methods CIK cells were cultured as effector cells and lung cancer cells were used as target cells. Co culture system between CIK cells and LLC mice lung cancer cell lines was established by co-culture of CIK cells and LLC mice lung cancer cell lines. The survival rate of lung cancer cells in LLC mice was detected by MTT colorimetry. Western blot was used to detect the expression of 14-3-3 味 -p-Bad protein in CIK cells. Results CIK cells were successfully induced under light microscope. The survival rate of LLC lung cancer cells cultured for 20 days was significantly lower than that in 10 d and 15 d groups. Western blot was used to detect the down-regulation of 14-3-3 味 -p-Bad expression in LLC mice lung cancer cells. Conclusion with the extension of culture time, the survival rate of CIK cells on lung cancer cells of LLC mice was decreased gradually and the apoptosis of lung cancer cells of LLC mice was promoted.
【作者单位】：锦州医科大学附属第三医院胸外科;锦州医科大学基础医学院;吉林省肿瘤医院胸外一科;锦州医科大学生命科学研究院;
【基金】：辽宁省教育厅2016年省级大学生创新创业训练计划项目(20161060044)
【分类号】：R734.2

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