中国尤文肉瘤患者EZH2蛋白表达与临床病理学参数及预后的关系

发布时间：2018-05-08 20:09

本文选题：zeste增强子同源物 + Ki-　；参考：《山东大学学报(医学版)》2017年02期

【摘要】：目的探讨果蝇zeste增强子同源物2(EZH2)蛋白在中国尤文肉瘤(EWS)患者中的表达与临床病理参数特征及预后的关系。方法收集67例尤文肉瘤/原始神经外胚层肿瘤(EWS/PNET)和其他小圆细胞恶性肿瘤石蜡标本并构建组织芯片,采用荧光原位杂交(FISH)法检测尤文肉瘤断裂位点1(EWSR1)基因易位;应用免疫组织化学法检测肿瘤组织标本中EZH2、Ki-67和P53的表达;应用Kaplan-Meier(K-M)和Cox多因素分析法进行生存分析。结果确诊EWS石蜡组织标本44例;EZH2蛋白在EWS组织中的高表达与肺转移及预后密切相关(P=0.023,P=0.004),并且与Ki-67表达呈正相关性(R=0.406,P=0.006)。EZH2高表达和高Ki-67增殖指数与患者不良预后呈显著相关性(P=0.006,P=0.023);Cox多因素分析表明,P53的表达与EWS患者的总体生存差异无统计学意义(P0.05),但EZH2和Ki-67是影响患者总体生存的独立预后判定因子(HR=3.467,95%CI=1.138~10.563,P=0.029;HR=2.140,95%CI=0.439~10.423,P=0.046)。进一步分析显示,EZH2和Ki-67同时高表达的亚群生存期最短(P=0.012)。结论 EZH2和Ki-67的表达是EWS患者的独立预后因素,并且EZH2和Ki-67的联合检测可以识别预后极差的一部分EWS患者,EZH2可能是EWS新的预后标志物。
[Abstract]:Objective to investigate the relationship between the expression of zeste enhancer 2EZH2 protein in Ewing's sarcoma (EWS) patients in China and the clinicopathological parameters and prognosis. Methods paraffin specimens from 67 Ewing sarcoma / primitive neuroectodermal tumors (EWS / PNET) and other small round cell malignant tumors were collected, and tissue microarray was constructed. Ewing sarcoma cleavage site 1EWSR1 gene translocation was detected by fluorescence in situ hybridization (fish). Immunohistochemical method was used to detect the expression of EZH2KI-67 and p53, and Kaplan-Meierer K-M) and Cox multivariate analysis were used to analyze the survival. Results the high expression of EZH2 protein in 44 EWS paraffin tissues was closely related to lung metastasis and prognosis, and positively correlated with the expression of Ki-67. The high expression of EZH2 and high Ki-67 proliferation index were significantly associated with the poor prognosis of the patients. Multivariate analysis showed that there was no significant difference between the expression of p53 and the overall survival of patients with EWS. However, EZH2 and Ki-67 were independent prognostic factors affecting the overall survival of patients. Further analysis showed that both EZH2 and Ki-67 were highly expressed in the subgroup with the shortest survival time (P = 0.012). Conclusion the expression of EZH2 and Ki-67 is an independent prognostic factor in EWS patients, and the combined detection of EZH2 and Ki-67 may be a new prognostic marker of EWS in EWS patients with poor prognosis.
【作者单位】：山东大学医学院病理学教研室;山东大学齐鲁医院病理科;
【基金】：国家自然科学基金(81472417,81528015) 山东省自然科学基金(21300005071402,26010105201308)
【分类号】：R738

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