小细胞肺癌相关血管信号通路的研究

发布时间：2018-05-08 17:40

本文选题：小细胞肺癌 + VEGF　；参考：《青岛大学》2015年硕士论文

【摘要】：目的对45例小细胞肺癌(SCLC)肿瘤组织和20例癌旁正常肺组织进行免疫组化实验,检测组织中相关血管信号因子如血管内皮生长因子(VEGF)、DLL4及Notch1的表达情况并进行比较,从而阐明信号因子之间的相互作用,为临床筛选抗血管生成药物提供理论依据。方法收集青岛市市立医院病理科2009年至2014年SCLC术后的存档蜡块共45例,患者术前均未经历放疗和化疗,术后诊断均经过免疫组化检测CD56、Syn、CK和NSE四项指标证实。其中男28例,女17例;最高年龄77岁,最低年龄29岁。同时选取癌旁正常肺组织蜡块20例作为对照。采用免疫组化法测量肿瘤组织和正常肺组织中VEGF、DLL4及Notch1蛋白的表达水平,显微镜下观察结果并记录阳性表达率。应用SPSS15.0软件对数据进行统计学分析,实验组与对照组间数据的比较采用χ2检验,实验组各指标间的相关性采用Spearman等级相关分析,P0.05时表示差异具统计学意义。结果VEGF、DLL4在SCLC肿瘤组织中的阳性率分别为71.1%和62.2%,均明显高于癌旁正常组织(分别为0和10%)(P0.01);Notch1在正常组织中的表达率(25%)略高于肿瘤组织(8.9%),两者无显著性差异(P0.05)。在SCLC肿瘤组织中VEGF与DLL4的表达呈高度正相关(rs=0.867,P0.01),Notch1与VEGF的表达呈负相关(rs=-0.425,P0.05),Notch1与DLL4的表达亦呈负相关(rs=-0.370,P0.05)。结论大多数SCLC的肿瘤组织中均存在VEGF、DLL4的表达,二者相互协同,共同促进肿瘤的生长;Notch1在正常肺组织中的表达略高于肿瘤组织,有抑制肿瘤生长的趋势;VEGF、DLL4及Notch1三者之间存在相关性。
[Abstract]:Objective to detect and compare the expression of vascular signal factors such as vascular endothelial growth factor (VEGF) DLL4 and Notch1 in 45 cases of small cell lung cancer (SCLC) tumor and 20 cases of adjacent normal lung tissues. Thus, the interaction between signal factors was elucidated and the theoretical basis for clinical screening of anti-angiogenic drugs was provided. Methods A total of 45 cases of archived wax masses after SCLC operation were collected from 2009 to 2014 in Department of Pathology of Qingdao City Hospital. All the patients were not treated with radiotherapy and chemotherapy before operation. The postoperative diagnosis was confirmed by immunohistochemical examination of CD56 and NSE. There were 28 males and 17 females, the highest age was 77 years and the minimum age was 29 years. At the same time, 20 cases of normal lung tissue adjacent to cancer were selected as control. The expression of VEGF DLL4 and Notch1 protein in tumor tissues and normal lung tissues were measured by immunohistochemical method. The results were observed under microscope and the positive rate was recorded. The data were analyzed by SPSS15.0 software. 蠂 2 test was used to compare the data between the experimental group and the control group. The correlation of each index in the experimental group was analyzed by Spearman grade correlation analysis (P0.05). Results the positive rates of VEGF DLL4 in SCLC tumor tissues were 71.1% and 62.2%, respectively, which were significantly higher than those in adjacent normal tissues (0 and 100.01% respectively) and slightly higher than those in normal tissues (P 0.05). There was a high positive correlation between the expression of VEGF and DLL4 in SCLC tumor tissues. There was a negative correlation between the expression of VEGF and DLL4 and the expression of VEGF. There was also a negative correlation between the expression of VEGF and the expression of DLL4. Conclusion the expression of VEGF DLL4 in most tumor tissues of SCLC is a little higher than that in normal lung tissues, and the expression of VEGF DLL4 is a little higher than that in normal lung tissues, and the expression of VEGF DLL4 is synergistic with each other. There was a tendency to inhibit tumor growth and there was a correlation between VEGF DLL4 and Notch1.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R734.2

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