基于聚多巴胺纳米材料的制备及在肿瘤微波热疗中的应用

发布时间：2018-05-09 14:28

本文选题：聚多巴胺 + 微波热疗　；参考：《北京交通大学》2016年硕士论文

【摘要】：近年来,肿瘤热疗技术的发展,显示出在肿瘤治疗方面的优势,其治疗方法简单,效果显著,无明显毒副作用,具有微创性等特点,因而被称为“绿色疗法”。其中,肿瘤微波热疗法,是一种内源性加热方式对生物体进行加热,它具有热转换效率高,穿刺深度大,热场均匀,凝固区域内坏死彻底和可操作性强等优点已逐渐成为治疗肿瘤的重要手段之一,为癌症患者带来了曙光。聚多巴胺(PDA),一种广泛分布在人体和生物体的物质,拥有许多独特的性能,已成为近年来的研究热点,广泛应用于能源材料、污水处理、传感器、药物运载等方面,特别是其优良的生物相容性,在生物医药领域表现出巨大的应用前景。本研究设计了一种具有良好生物相容性的微波增敏性物质,一方面提高微波热转换效率,另一方面增强热疗过程中的靶向性,同时采取热疗与化疗结合的方式进行了研究,进一步实现了低功率高效率的肿瘤微波热疗。主要工作内容如下：一、采用模板法和物理灌装法合成了PDA包覆离子液体(ILs)的纳米复合物。ILs/PDA纳米复合物由于PDA壳层的限域效应,与游离态的离子液体相比,表现出更迅速、更特定、更安全的微波升温效果。体外细胞实验,体内急性毒性实验都表明其良好的生物相容性。此外,将ILs/PDA纳米复合物应用于ICR小鼠活体模型的微波热疗实验,结果发现治疗组的肿瘤得到有效抑制,而且,ILs/PDA纳米复合物,在采用尾静脉给药方式时,表现出显著的靶向作用。二、以ILs/PDA纳米复合物为前驱体,采用物理震荡法合成了离子液体-聚多巴胺-阿霉素(IL-PDA-DOX)纳米复合物,提供了一种化疗与热疗相结合的治疗平台。体外升温实验证明了材料的微波增敏性能。体内外毒性实验评价了材料的安全性。通过降低微波热疗功率,将IL-PDA-DOX纳米复合物应用于ICR小鼠活体模型,得到优异的抑瘤效果。
[Abstract]:In recent years, the development of tumor hyperthermia technology has shown its advantages in tumor treatment. Its treatment method is simple, effective, has no obvious toxic side effects, and has the characteristics of minimally invasive, so it is called "green therapy". Among them, tumor microwave thermotherapy is an endogenous heating way to heat organisms, it has a high efficiency of heat conversion, puncture depth, uniform thermal field, The advantages of complete necrosis and strong maneuverability in coagulation area have gradually become one of the important methods for the treatment of cancer, which brings dawn to cancer patients. Polydopamine (PDA), a kind of substance widely distributed in human body and organism, with many unique properties, has become a research hotspot in recent years. It has been widely used in energy materials, sewage treatment, sensors, drug delivery and so on. Especially its excellent biocompatibility shows great application prospect in the field of biomedicine. In this study, a kind of microwave sensitizing substance with good biocompatibility was designed. On the one hand, the efficiency of microwave heat transfer was improved, on the other hand, the targeting of hyperthermia was enhanced. At the same time, the combination of hyperthermia and chemotherapy was adopted. Furthermore, low power and high efficiency microwave hyperthermia is realized. The main work is as follows: first, the nanocomposites coated with PDA / ils were synthesized by template method and physical filling method. Because of the limiting effect of PDA shell, the nanocomposites showed faster than the free ionic liquids. More specific, safer microwave heating effect. Both in vitro cell test and in vivo acute toxicity test showed its good biocompatibility. In addition, the ILs/PDA nanocomposites were used in the microwave hyperthermia of ICR mice model in vivo. The results showed that the tumor of the treatment group was effectively inhibited, and the Nano complexes of ils / PDAs showed a significant targeting effect when the drug was administered via caudal vein. Secondly, using ILs/PDA nanocomposites as precursors, the ionic liquid-polydopamine doxorubicin IL-PDA-DOX nanocomposites were synthesized by physical shock method, which provided a therapeutic platform for the combination of chemotherapy and hyperthermia. The microwave sensitizing property of the material was proved by in vitro heating test. In vivo and in vitro toxicity experiments were used to evaluate the safety of the materials. By reducing the power of microwave hyperthermia and applying IL-PDA-DOX nanocomposites to ICR mice in vivo, excellent tumor inhibition effect was obtained.
【学位授予单位】：北京交通大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：TB383.1;R730.5

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